論文

• Zihan Qie, Kazuho Kosuge, Tetsuo Sakurai, Sivashankari M Ramamoorthi, Yuki Miyahara, Takeharu Tsuge. Biodegradability of oxidized films of polyhydroxyalkanoate copolymers containing 2-hydroxy-4-methylthiobutyrate unit in seawater, Polym Degrad Stabil, 229, 110975, Sept. 2024.
• Chih-Ting Wang, Ramamoorthi M Sivashankari, Yuki Miyahara, Takeharu Tsuge. Polyhydroxyalkanoate copolymer production by recombinant Ralstonia eutropha strain 1F2 from fructose or carbon dioxide as sole carbon source, Bioengineering, Vol. 11, No. 5, 455, May 2024.
• Maierwufu Mierzati, Yuki Miyahara, Blanche Curial, Christopher T. Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Tacticity Characterization of Biosynthesized Polyhydroxyalkanoates Containing (S)- and (R)-3-Hydroxy-2-Methylpropionate Units, Biomacromolecules, 25, 444−454, Jan. 2024.
• Yuki Miyahara, Marii Ishino, Christopher T. Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Biosynthesis of polyhydroxyalkanoate copolymers consisting of α-methylated monomer units from glucose and propionate: thermal properties and chiral configuration, Biotechnol. Sustainable Mater., 2024.
• Maierwufu Mierzati, Tetsuo Sakurai, Manami Ishii-Hyakutake, Yuki Miyahara, Christopher T Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Biosynthesis, characterization, and biodegradation of elastomeric polyhydroxyalkanoates consisting of α-dimethylated monomer units, Mater. Today Sustain., 24, 100577, Dec. 2023.
• Ramamoorthi M. Sivashankari, Yuki Miyahara, Takeharu Tsuge. Poly(3-hydroxybutyrate) Biosynthesis from [U-13C6]D-Glucose by Ralstonia eutropha NCIMB 11599 and Recombinant Escherichia coli, Microbiol. Res., Vol. 14, No. 4, pp. 1894-1906, Nov. 2023.
• Shin-Ichi Hachisuka, Tetsuo Sakurai, Shoji Mizuno, Kazuho Kosuge, Sayaka Endo, Manami Ishii-Hyakutake, Yuki Miyahara, Motoyuki Yamazaki, Takeharu Tsuge. Isolation and characterization of polyhydroxyalkanoate-degrading bacteria in seawater at two different depths from Suruga Bay, Appl. Environ. Microbiol., Vol. 89, No. 11, e0148823, Nov. 2023.
• Yuki Miyahara, Tomoya Nakamura, Maierwufu Mierzati, Zihan Qie, Tomoki Shibasaka, Christopher T. Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Thermal and crystallization properties of a polyhydroxyalkanoate binary copolymer containing 3-hydroxybutyrate and 3-hydroxy-2-methylvalerate units, Processes, Vol. 11, No. 7, 1901, June 2023.
• Ramamoorthi M. Sivashankari, Maierwufu Mierzati, Yuki Miyahara, Shoji Mizuno, Christopher T. Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Exploring Class I polyhydroxyalkanoate synthases with broad substrate specificity for polymerization of structurally diverse monomer units, Front. Bioeng. Biotechnol., 11, 1114946, Feb. 2023.
• Shoji Mizuno, Ayata Nakagawa, Tetsuo Sakurai, Yuki Miyahara, Takeharu Tsuge. Oxidation of methionine-derived 2-hydroxyalkanoate unit in biosynthesized polyhydroxyalkanoate copolymers, Int J Biol Macromol, Vol. 224, pp. 840-847, Jan. 2023.
• Yuki Miyahara, Chih-Ting Wang, Manami Ishii-Hyakutake, Takeharu Tsuge. Continuous supply of non-combustible gas mixture for safe autotrophic culture to produce polyhydroxyalkanoate by hydrogen-oxidizing bacteria, Bioengineering, 9, 586, Oct. 2022.
• Ceneviva Lucas V.S., Maierwufu Mierzati, Yuki Miyahara, Christopher T. Nomura, Seiichi Taguchi, Hideki Abe, Takeharu Tsuge. Poly(3-mercapto-2-methylpropionate), a novel α-methylated bio-polythioester with rubber-like elasticity, and its copolymer with 3-hydroxybutyrate: biosynthesis and characterization, Bioengineering, Vol. 9, No. 5, 228, May 2022.
• Shu Saito, Imai, R., Yuki Miyahara, Nakagawa, M., Orita, I., Tsuge, T., Toshiaki Fukui. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose by Escherichia coli through butyryl-CoA formation driven by Ccr-Emd combination, Frontiers in Bioengineering and Biotechnology, Vol. 10, Apr. 2022.  公式リンク  
  
• Saki Goto, Yuki Miyahara, Seiichi Taguchi, Takeharu Tsuge, Ayaka Hiroe. Enhanced production of (R)-3-hydroxybutyrate oligomers by coexpression of molecular chaperones in recombinant Escherichia coli harboring a polyhydroxyalkanoate synthase derived from Bacillus cereus YB-4, Microorganisms, Vol. 10, No. 2, 458, Feb. 2022.
• Tetsuo Sakurai, Shoji Mizuno, Yuki Miyahara, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge. Optimization of culture conditions for secretory production of 3-hydroxybutyrate oligomers using recombinant Escherichia coli, Frontiers in Bioengineering and Biotechnology, Vol. 10, 829134, Feb. 2022.
• Hua Tiang Tan, Min Fey Chek, Yuki Miyahara, Sun-Yong Kim, Takeharu Tsuge, Toshio Hakoshima, Kumar Sudesh. Characterization of an (R)-specific enoyl-CoA hydratase from Streptomyces sp. strain CFMR 7: A metabolic tool for enhancing the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), J. Biosci. Bioeng., Vol. 134, No. 4, pp. 288-294, 2022.
• Fakhrul Ikhma Mohd Fadzil, Makoto Kobayashi, Yuki Miyahara, Manami Ishii-Hyakutake, Takeharu Tsuge. Biosynthesis of poly(3-hydroxybutyrate-co‑3-hydroxyhexanoate) with a high 3-hydroxyhexanoate fraction and low molecular weight for polymer blending, Journal of Siberian Federal University. Biology., Vol. 14, No. 4, pp. 442–453, Dec. 2021.
• Romeo Thorbecke, Masahiro Yamamoto, Yuki Miyahara, Mino Oota, Shoji Mizuno, Takeharu Tsuge. The gene dosage effect of carbonic anhydrase on the biosynthesis of poly(3-hydroxybutyrate) under autotrophic and mixotrophic culture conditions, Polym J, Vol. 53, pp. 209–213, Jan. 2021.
• Ayaka Hiroe, Tetsuo Sakurai, Shoji Mizuno, Yuki Miyahara, Saki Goto, Mariko Yamada, Takeharu Tsuge, Seiichi Taguchi. Microbial oversecretion of (R)-3-hydroxybutyrate oligomer with diethylene glycol terminal as a macromonomer for polyurethane synthesis, Int J Biol Macromol, Vol. 167, pp. 1290-1296, Jan. 2021.
• Yuki Miyahara, Masahiro Yamamoto, Romeo Thorbecke, Shoji Mizuno, Takeharu Tsuge. Autotrophic biosynthesis of polyhydroxyalkanoate by Ralstonia eutropha from non-combustible gas mixture with low hydrogen content, Biotechnol. Lett., 42, pp. 1655–1662, Sept. 2020.
• Yuki Miyahara, Ayaka Hiroe, Takeharu Tsuge, Seiichi Taguchi. Microbial secretion platform for 3-hydroxybutyrate oligomers and its end-capped forms using chain transfer reaction-mediated polyhydroxyalkanoate synthases, Biotechnol. J., Vol. 14, No. 12, Dec. 2019.
• Yuki Miyahara, Mino Oota, Takeharu Tsuge. NADPH supply for poly(3-hydroxybutyrate) synthesis concomitant with enzymatic oxidation of phosphite, J. Biosci. Bioeng., Vol. 126, No. 6, pp. 764-768, Dec. 2018.
• Ayaka Hokamura, Izumi Wakida, Yuki Miyahara, Takeharu Tsuge, Hideki Shiratsuchi, Kenji Tanaka, Hiromi Matsusaki. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyalkanoates) by recombinant Escherichia coli from glucose, J. Biosci. Bioeng., Vol. 120, No. 3, pp. 305-310, Sept. 2015.

著書

• 宮原佑宜, 柘植丈治. 第2章 水素酸化細菌による生分解性ポリエステル生産, 独立栄養微生物によるCO2資源化技術, シーエムシー出版, Dec. 2023.
• 宮原佑宜, 柘植丈治. 第Ⅰ編 生合成 第2章 中鎖長PHAホモポリマーおよび分岐側鎖PHA共重合体の生合成, 微生物産生ポリエステルの基礎と応用, シーエムシーリサーチ, Nov. 2023.
• 宮原佑宜, 柘植丈治. 第11章 ポリヒドロキシアルカン酸(PHA), プラスチック海洋問題と対応策, CMC出版, Jan. 2023.
• 宮原 佑宜, 柘植丈治. 第６章ポリヒドロキシアルカン酸（PHA）, バイオプラスチックの最新技術動向 ―真の普及を目指して―, CMC出版, July 2022.
• 宮原佑宜, 柘植丈治. 第８章４節 ポリヒドロキシアルカン酸の微生物合成, バイオリアクターのスケールアップと物質生産事例集, エヌ・ティー・エス, Apr. 2021.
• Yuki Miyahara, Ayaka Hiroe, Shunsuke Sato, Takeharu Tsuge, Seiichi Taguchi. Chapter 8: Microbial Polyhydroxyalkanoates (PHAs): From Synthetic Biology to Industrialization, Biopolymers for Biomedical and Biotechnological Applications, Wiley, pp. 231-264, 2020.

特許など

• 柘植丈治, MRAMAMOORTHI Sivashankari, 宮原佑宜, 山本　智義, 小澤　優. ポリヒドロキシアルカン酸の製造方法. 特許. 公開. 国立大学法人東京工業大学, 帝人株式会社. 2023/10/12. PCT/JP2023/037027. 2024/05/16. WO 2024/101063. 2024.
• 柘植丈治, 水野匠詞, 廣江 綾香, 宮原佑宜, 田口 精一. ポリヒドロキシアルカン酸オリゴマーの製造方法. 特許. 公開. 国立大学法人東京工業大学, 学校法人東京農業大学. 2020/02/28. 特願2020-032735. 2020/09/10. 特開2020-141665. 2020. Sept.

学位論文

• 補酵素再生系の強化によるポリヒドロキシアルカン酸の生合成,  審査の要旨,  博士(工学),  東京工業大学,  2018/09/20,   
• 補酵素再生系の強化によるポリヒドロキシアルカン酸の生合成,  論文要旨,  博士(工学),  東京工業大学,  2018/09/20,   
• 補酵素再生系の強化によるポリヒドロキシアルカン酸の生合成,  要約,  博士(工学),  東京工業大学,  2018/09/20,   
• 補酵素再生系の強化によるポリヒドロキシアルカン酸の生合成,  本文,  博士(工学),  東京工業大学,  2018/09/20, 

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