Japanese Society for Plant Cell and Molecular Biology
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In present-day biology, a facile gene delivery system is required to study gene function in a high throughput way. The use of plant virus vectors that can introduce foreign or extra endogenous genes into plants attracts much attention. It is advantageous in that researchers need only minimal experience and results can be obtained in a short period of time. Here we describe infectious cDNA clones of the tomato mosaic tobamoviruses (ToMV) that were modified for facile insertion of foreign genes and which retained the ability to multiply in plants. This vector, named TocJ, was able to harbor the GFP gene from Aequorea victoria and systemically spread in some Solanaceous plants. TocJ would be suitable for the overexpression of foreign genes and the analysis of gene functions in various Solanaceous plants.
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