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タイトル
和文: 
英文:Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning 
著者
和文: 菅原 路子, 三好 洋美, 三浦 拓也, 田中 博人, 坪田 健一, 劉 浩.  
英文: Michiko Sugawara, Hiromi Miyoshi, Takuya Miura, Hiroto Tanaka, Ken-ichi Tsubota, Hao Liu.  
言語 English 
掲載誌/書名
和文: 
英文:BioMed Research International 
巻, 号, ページ vol. 2016        pp. 1-16
出版年月 2016年12月31日 
出版者
和文: 
英文:Hindawi Publishing 
会議名称
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英文: 
開催地
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英文: 
公式リンク https://www.hindawi.com/journals/bmri/2016/5749749/
 
DOI http://dx.doi.org/10.1155/2016/5749749
アブストラクト To understand the mechanism regulating the spontaneous change in polarity that leads to cell turning, we quantitatively analyzed the dynamics of focal adhesions (FAs) coupling with the self-assembling actin cytoskeletal structure in Swiss 3T3 fibroblasts. Fluorescent images were acquired from cells expressing GFP-actin and RFP-zyxin by laser confocal microscopy. On the basis of the maximum area, duration, and relocation distance of FAs extracted from the RFP-zyxin images, the cells could be divided into 3 regions: the front region, intermediate lateral region, and rear region. In the intermediate lateral region, FAs appeared close to the leading edge and were stabilized gradually as its area increased. Simultaneously, bundled actin stress fibers (SFs) were observed vertically from the positions of these FAs, and they connected to the other SFs parallel to the leading edge. Finally, these connecting SFs fused to form a single SF with matured FAs at both ends. This change in SF organization with cell retraction in the first cycle of migration followed by a newly formed protrusion in the next cycle is assumed to lead to cell turning in migrating Swiss 3T3 fibroblasts.

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