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タイトル
和文: 
英文:Recruitment and delivery of the fission yeast Rst2 transcription factor via a local genome structure counteracts repression by Tup1-family corepressors 
著者
和文: Ryuta Asada, Miki Umeda, Akira Adachi, Satoshi Senmatsu, Takuya Abe, 岩崎 博史, Kunihiro Ohta, Charles S. Hoffman, Kouji Hirota.  
英文: Ryuta Asada, Miki Umeda, Akira Adachi, Satoshi Senmatsu, Takuya Abe, Hiroshi Iwasaki, Kunihiro Ohta, Charles S. Hoffman, Kouji Hirota.  
言語 English 
掲載誌/書名
和文:Nucleic Acids Research 
英文:Nucleic Acids Research 
巻, 号, ページ Vol. 45    No. 16    pp. 9361-9371
出版年月 2017年9月 
出版者
和文: 
英文:Oxford University Press 
会議名称
和文: 
英文: 
開催地
和文: 
英文: 
ファイル
公式リンク http://www.scopus.com/inward/record.url?eid=2-s2.0-85031931694&partnerID=MN8TOARS
 
DOI https://doi.org/10.1093/nar/gkx555
アブストラクト Transcription factors (TFs) determine the transcription activity of target genes and play a central role in controlling the transcription in response to various environmental stresses. Three dimensional genome structures such as local loops play a fundamental role in the regulation of transcription, although the link between such structures and the regulation of TF binding to cis-regulatory elements remains to be elucidated. Here, we show that during transcriptional activation of the fission yeast fbp1 gene, binding of Rst2 (a critical C2H2 zinc-finger TF) is mediated by a local loop structure. During fbp1 activation, Rst2 is first recruited to upstream-activating sequence 1 (UAS1), then it subsequently binds to UAS2 (a critical cis-regulatory site located approximately 600 base pairs downstream of UAS1) through a loop structure that brings UAS1 and UAS2 into spatially close proximity. Tup11/12 (the Tup-family corepressors) suppress direct binding of Rst2 to UAS2, but this suppression is counteracted by the recruitment of Rst2 at UAS1 and following delivery to UAS2 through a loop structure. These data demonstrate a previously unappreciated mechanism for the recruitment and expansion of TF-DNA interactions within a promoter mediated by local three-dimensional genome structures and for timely TF-binding via counteractive regulation by the Tup-family corepressors.

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