論文

• Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Mitsue Nagamine, Hiroshi Ueda, Tetsuya Kitaguchi. Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody, Biosensors and Bioelectronics, 267, 116796, Sept. 2024.
• Kana Sasamoto, Takanobu Yasuda, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi. Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M, The Analyst, vol. 148, p. 5843-5850, Oct. 2023.  公式リンク  
  
• Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi. Development of green fluorescent protein-based cAMP indicators for covering a wide range of cAMP concentrations., RSC Advances, 13, 15514-15520, May 2023.
• Yoshihiro Ito, Ryuichi Sasaki, Sayaka Asari, Takanobu Yasuda, Hiroshi Ueda, Tetsuya Kitaguchi. Efficient Microfluidic Screening Method Using a Fluorescent Immunosensor for Recombinant Protein Secretions, Small, Volume 19, Issue 34, 2207943, Apr. 2023.  公式リンク  
  
• Satoshi Okada, Yuka Muto, Bo Zhu, Hiroshi Ueda, Hiroyuki Nakamura. Development of a Peptide Sensor Derived from Human ACE2 for Fluorescence Polarization Assays of the SARS-CoV-2 Receptor Binding Domain, Analytical Chemistry, 95, 6198-6202, Apr. 2023.  公式リンク  
  
• Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda. Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces, Biochemistry, Jan. 2023.
• Jiulong Su, Bo Zhu, Akihito Inoue, Hiroyuki Oyama, Izumi Morita, Jinhua Dong, Takanobu Yasuda, Yoshiko Sugita-Konishi, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda. The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning, Biosensors and Bioelectronics, Elsevier, Vol. 219, 114793, Jan. 2023.
• B. Zhu, N. Nosaka, S. Kanamaru, J. Dong, Y. Dai, A. Inoue, Y. Yang, K. Kobayashi, T. Kitaguchi, H. Iwasaki, R. Koike, K. Wakabayashi, H. Ueda. Rapid and sensitive SARS-CoV-2 detection using a homogeneous fluorescent immunosensor Quenchbody with crowding agents, Analyst, Vol. 147, Number 22, p. 4971-4979, Nov. 2022.  公式リンク  
  
• Mai Takizawa, Yuri Osuga, Rika Ishida, Marie Mita, Kazuki Harada, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi.. Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging., Commun Biol, 5, 833, Sept. 2022.
• Haimei Li, Bo Zhu, Baowei Li, Limei Chen, Xuerao Ning, Hang Dong, Jingru Liang, Xueying Yang, Jinhua Dong, Hiroshi Ueda. Isolation of a human SARS-CoV-2 neutralizing antibody from a synthetic phage library and its conversion to fluorescent biosensors, Scientific Reports, Springer Nature, vol. 12, p. 15496, Sept. 2022.
• Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimura, Farid J. Ghadess, Hiroshi Ueda. Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting, Chemical Science, The Royal Society of Chemistry, Vol. 13, Issue 33, p. 9739-9748, Aug. 2022.
• I. Morita, Y. Kiguchi, H. Oyama, K. Yamaki, N. Sakio, Keisuke Kashiwabara, Y. Kuroda, A. Ito, A. Yokota, N. Ikeda, A. Takeuchi, C. Tode, H. Ueda, S. Numazawa, R. Kikura-Hanajiri, T. Yoshida, N. Kobayashi. Derivatization-assisted immunoassays: application for group-specific detection of potent methamphetamine and amphetamine enantiomers, Analytical Methods, July 2022.
• H.-O. Yun, H. Ueda, H.-J. Jeong. Development of a spacer-optimized Quenchbody against tumor necrosis factor alpha, Biotechnology annd Bioprocess Engineering, July 2022.
• J. Liang, H. Dong, H. Wang, G. Jiang, T. Inagaki, C.E. Gomez-Sanchez, J. Liang, H. Dong, H. Wang, Z. Yi, G. Jiang, T. Inagaki, C.E. Gomez-Sanchez, J. Dong*, H. Ueda*. Creation of a Quick and Sensitive Fluorescent Immunosensor for Detecting the Mineralocorticoid Steroid Hormone Aldosterone, Journal of Steroid Biochemistry and Molecular Biology, Apr. 2022.
• Yuki Ohmuro-Matsuyama, Tadaomi Furuta, Hayato Matsui, Masaki Kanai, Hiroshi Ueda. Miniaturization of bright light-emitting luciferase ALuc: picALuc, ACS Chem. Biol., vol. 17, no. 4, pp. 864-872, Mar. 2022.
• Marie Mita, Izumi Sugawara, Kazuki Harada, Motoki Ito, Mai Takizawa, Kentaro Ishida, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi.. Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics, Cell Chem Biol, 29, 98, Jan. 2022.
• H. Li, X. Li, H. Li, H. Dong, X. Yang, H. Ueda, J. Dong. Quench-release-based Fluorescent Immunosensor for the Rapid Detection of Tumor Necrosis Factor-alpha, ACS Omega, Nov. 2021.
• Yuki Ohmuro-Matsuyama, Keiko Gomi, Takuya Shimoda, Hideki Yamaji, Hiroshi Ueda. Improving the stability of protein–protein interaction assay FlimPIA using a thermostabilized firefly luciferase, Frontiers in Bioengineering and Biotechnology, Frontiers Media, Vol. 9, p. 778120, Nov. 2021.
• Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami, Hiroshi Ueda. Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface, Scientific Reports, Springer Nature, Vol. 11, p. 22590, Nov. 2021.
• Shinichi Sato, Masaki Matsumura, Hiroshi Ueda, Hiroyuki Nakamura. Preparation of an antigen-responsive fluorogenic immunosensor by tyrosine chemical modification of the antibody complementarity determining region, Chemical Communications, RSC, Aug. 2021.
• Xuerao Ning, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda. Construction of Fluorescent Immunosensor Quenchbody to Detect His-Tagged Recombinant Proteins Produced in Bioprocess, Sensors, MDPI, vol. 21, Issue 15, p. 4993, July 2021.  公式リンク  
  
• T. Yasuda, A. Inoue, T. Kitaguchi, H. Ueda. Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides, Chemical Communications, vol. 57, p. 8206-8209, July 2021.  公式リンク  
  
• Y. Liang, Y. Wang, F. Wang, J. Li, C. Wang, J. Dong, H. Ueda, Z. Xiao, Y. Shen, Z. Xu, H. Wang. An Enhanced Open Sandwich Immunoassay by Molecular Evolution for Noncompetitive Detection of Alternaria Mycotoxin Tenuazonic Acid, Food Chemistry, Elsevier, Vol. 361, 130103, May 2021.
• Riho Takahashi, Takanobu Yasuda, Yuki Ohmuro-Matsuyama, Hiroshi Ueda. BRET Q‐Body: A Ratiometric Quench-based Bioluminescent Immunosensor Made of Luciferase−Dye−Antibody Fusion with Enhanced Response, Analytical Chemistry, Vol. 93, no. 21, p. 7571-7578, May 2021.
• Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Hiroshi Kimura, Hiroshi Ueda. Simple Fluorogenic Cellular Assay for Histone Deacetylase Inhibitors Based on Split-Yellow Fluorescent Protein and Intrabodies, ACS Omega, American Chemical Society, vol. 6, Issue 15, p. 10039-10046, Apr. 2021.
• Hiroto Okuyama, Takanori Tamaki, Yuhei Oshiba, Hiroshi Ueda, Takeo Yamaguchi. Numerical Modeling for Sensitive and Rapid Molecular Detection by Membrane-Based Immunosensors, Analytical Chemistry, vol. 93, 19, 7210-7219, Mar. 2021.
• Akihito Inoue, Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Hiroshi Ueda. Creation of a Nanobody-Based Fluorescent Immunosensor Mini Q‐body for Rapid Signal-On Detection of Small Hapten Methotrexate, ACS Sensors, vol. 5, no. 11, p. 3457–3464, Nov. 2020.  公式リンク  
  
• Yoshitaka HARADA, Yuki OHMURO-MATSUYAMA, Mika TSUNA, Hiroshi UEDA. An Open Sandwich Immunochromatography for Non-competitive Detection of Small Antigens, Analytical Sciences, Japan Society for Analytical Chemistry, Vol. 37, No. 3, p. 455-459, Nov. 2020.
• Kazuki Harada, Takami Chihara, Yuki Hayasaka, Marie Mita, Mai Takizawa, Kentaro Ishida, Mary Arai, Saki Tsuno, Mitsuharu Matsumoto, Takeshi Ishihara, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi. Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging., Sci Rep, 10, 19562, Nov. 2020.
• L. Chen, R. Tan, Y. Zhou, L. Zhang, S. Zhanga, X. Li, Y. Cong, H. Li, P. Sun, L. Chen, R. Tan, Y. Zhou, L. Zhang, S. Zhang, X. Li, Y. Cong, H. Li, P. Sun, H. Ueda, J. Dong. Development of an Open Sandwich ELISA for the Detection of Microcystin-LR, Microchemical Journal, vol. 158, p. 105325, July 2020.  公式リンク  
  
• J. Dong, C. Miyake, T. Yasuda, H. Oyama, I. Morita, T. Tsukahara, M. Takahashi, H.-J. Jeong, T. Kitaguchi, N. Kobayashi, H. Ueda. PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor, Biosensors and Bioelectronics, Elsevier, vol. 165, p. 112425, July 2020.  公式リンク  
  
• Hee-Jin Jeong, Jinhua Dong, Chang-Hun Yeom, Hiroshi Ueda. Synthesis of Quenchbodies for One-Pot Detection of Stimulant Drug Methamphetamine, Methods and Protocols, MDPI, Vol. 3, Issue 2, 43, June 2020.
• Chungyong Han, Beom K. Choi, Seon-Hee Kim, Su-Jung Sim, Seongeun Han, Bomi Park, Yohei Tsuchiya, Masaki Takahashi, Young H. Kim, Hyeon-Seok Eom, Tetsuya Kitaguchi, Hiroshi Ueda, Byoung S. Kwon. Polymorphic region-specific antibody for evaluation of affinity-associated profile of chimeric antigen receptor, Molecular Therapy: Oncolytics, Cell Press, Vol. 17, p. 293-305, Apr. 2020.  公式リンク  
  
• Shinichi Sato, Masaki Matsumura, Tetsuya Kadonosono, Satoshi Abe, Takafumi Ueno, Hiroshi Ueda, Hiroyuki Nakamura. Site-selective protein chemical modification of exposed tyrosine residues using tyrosine click reaction, Bioconjugate Chemistry, Mar. 2020.  公式リンク  
  
• Jinhua Dong, Yuya Oka, Hee-Jin Jeong, Yuki Ohmuro-Matsuyama, Hiroshi Ueda. Detection and destruction of HER2-positive cancer cells by Ultra Quenchbody-siRNA complex, Biotechnology and Bioengineering, Vol. 117, No. 5, p. 1259-1269, Feb. 2020.
• Kanai, Y., Ohmuro-Matsuyama, Y., Tanioku, M., Ushiba, S., Ono, T., Inoue, K., Kitaguchi, T., Kimura, M., Ueda, H., Matsumoto, K.. Graphene Field Effect Transistor-Based Immunosensor for Ultrasensitive Noncompetitive Detection of Small Antigens, ACS Sensors, Vol. 5, No. 1, p. 24-28, Jan. 2020.
• Jiulong Su, Tetsuya Kitaguchi, Yuki Ohmuro-Matsuyama, Theresa Seah, Farid J. Ghadessy, Shawn Hoon, Hiroshi Ueda. Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens, Scientific Reports, Springer-Nature, vol. 9, p. 18189, Dec. 2019.
• Y. Cong, H. Dong, X. Wei, L. Zhang, J. Bai, J. Wu, J.X. Huang, Z. Gao, H. Ueda, J. Dong. A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol, Ecotoxicology and Environmental Safety, Vol. 182, p. 109473, Oct. 2019.
• Chan-I Chung, Yuko Sato, Yuki Ohmuro-Matsuyama, Shinichi Machida, Hitoshi Kurumizaka, Hiroshi Kimura, Hiroshi Ueda. Intrabody-based FRET probe to visualize endogenous histone acetylation, Scientific Reports, Springer-Nature, Vol. 9, No. 1, pp. 10188, July 2019.  公式リンク  
  
• Jiulong Su, Cyrus Beh, Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Shawn Hoon, Hiroshi Ueda. Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens, Journal of Bioscience and Bioengineering, June 2019.
• Renee Dale, Yuki Ohmuro-Matsuyama, Hiroshi Ueda, Naohiro Kato. Non-steady state analysis of enzyme kinetics in real time elucidates substrate association and dissociation rates: demonstration with analysis of firefly luciferase mutants, BIOCHEMISTRY-US, vol. 58, no. 23, p. 2695-2702, May 2019.  公式リンク  
  
• Yuki Ohmuro-Matsuyama, Hiroshi Ueda. Protein-Protein Interaction Assays Using Split-NanoLuc, Bioluminescence - Analytical Applications and Basic Biology, IntechOpen, Apr. 2019.
• Marie Mita, Motoki Ito, Kazuki Harada, Izumi Sugawara, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi. Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells, Analytical Chemistry, Apr. 2019.  公式リンク  
  
• M. Mita, M. Ito, I. Sugawara, H. Ueda, T. Tsuboi, T. Kitaguchi. Green fluorescent protein-based glucose indicators report glucose dynamics in living cells, Analytical Chemistry, Vol. 91, No. 7, p. 4821-4830, Mar. 2019.
• Hee-Jin Jeong, Jinhua Dong, Hiroshi Ueda. Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies, Sensors, MDPI, vol. 19, Issue1, 52, Dec. 2018.
• A. Sasao, M. Takaki, H.-J. Jeong, K. Yonemitsu, Y. Ohtsu, H. Tsutsumi, S. Furukawa, H. Morioka, H. Ueda, Y. Nishitani. Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor, Drug Testing and Analysis, Vol. 11, p. 601-609, Oct. 2018.  公式リンク  
  
• Yuki Ohmuro-Matsuyama, Takahiro Yamashita, Keiko Gomi, Hideki Yamaji, Hiroshi Ueda. Evaluation of protein-ligand interactions using the luminescent interaction assay FlimPIA with streptavidin-biotin linkage, Analytcal Biochemistry, vol. 563, p. 61-66, Oct. 2018.  公式リンク  
  
• Jinhua Dong, Richi Fujita, Tamotsu Zako, Hiroshi Ueda. Construction of Quenchbodies to detect and image amyloid β oligomers, ANAL BIOCHEM, vol. 550, p. 61-67, Apr. 2018.
• Jiulong Su, Jinhua Dong, Tetsuya Kitaguchi, Yuki Omuro, Hiroshi Ueda. Noncompetitive homogeneous immunodetection of small molecules based on beta-glucuronidase complementation, Analyst, vol. 143, p. 2096-2101, Apr. 2018.
• Shitao Zhao, Jinhua Dong, Hee-Jin Jeong, Koichi Okumura, Hiroshi Ueda. Rapid detection of the neonicotinoid insecticide imidacloprid using a quenchbody assay, Analytical and Bioanalytical Chemistry, Apr. 2018.
• Yuki Omuro, H. Ueda. Homogeneous noncompetitive luminescent immunodetection of small molecules by ternary protein fragment complementation, Analytical Chemistry, vol. 90, no. 5, p. 3001-4, Feb. 2018.  公式リンク  
  
• Hee-Jin Jeong, Takuya Kawamura, Manami Iida, Yumi Kawahigashi, Mutsumi Takigawa, Yuki Ohmuro-Matsuyama, Chan-I Chung, Jinhua Dong, Masuo Kondoh, Hiroshi Ueda. Development of a Quenchbody for the detection and imaging of the cancer-related tight-junction-associated membrane protein claudin, Analytical Chemistry, American Chemical Society, Volume 89, Issue 20, Pages 10783-10789, Oct. 2017.
• Masayoshi Tanaka, Okochi M, Muto M, Yanai K, Onodera T, Wang J, Ueda H, Toko K.. Array-Based Rational Design of Short Peptide Probe-Derived from an Anti-TNT Monoclonal Antibody., ACS combinatorial science, Sept. 2017.  公式リンク  
  
• H.-J. Jeong, K. Matsumoto, S. Itayama, K. Kodama, R. Abe, J. Dong, M. Shindo, H. Ueda. Construction of dye-stapled Quenchbody by photochemical crosslinking to antibody nucleotide-binding site, Chemical Communications, Vol. 53, p. 10200-10203, Aug. 2017.
• Yuki Omuro, Takahiro Yamashita, Lin Huan, Hideki Yamaji, Hiroshi Ueda. Improved sensitivity of firefly luminescent intermediate-based protein interaction assay using Ser 440 mutant with lower adenylation activity, Luminescence, Wiley, Vol. 33, p. 125-130, July 2017.
• Devina Wongso, Jinhua Dong, Hiroshi Ueda, Tetsuya Kitaguchi. Flashbody: a next generation Fluobody with fluorescence intensity enhanced by antigen binding, Analytical Chemistry, Vol. 89, p. 6719-6725, May 2017.
• Y. Ohmuro, H. Ueda. Ultrasensitive Firefly luminescent intermediate-based Protein-protein Interaction Assay (FlimPIA) based on the functional complementation of mutant firefly luciferases, Methods in Molecular Biology, Springer New York, Vol. 1596, p. 119-130, Mar. 2017.  公式リンク  
  
• Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, Hiroshi Ueda. Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains, Methods in Molecular Biology, Springer New York, Vol. 1596, p. 149-165, Mar. 2017.  公式リンク  
  
• Jinhua Dong, Hiroshi Ueda. ELISA-type assays of trace biomarkers using microfluidic methods, WIREs Nanomed. Nanobiotechnol., Feb. 2017.
• Hiroshi Ueda. An Open Sandwich immunoassay for detection of 13(R,S)-Hydroxy-9(E),11(E)-octadecadienoic acid, Analyst, vol. 142, p. 787-793, Feb. 2017.  公式リンク  
  
• Hee-Jin Jeong, Tomoki Kojima, Jinhua Dong, Hiroyuki Ohashi, Hiroshi Ueda. One-pot construction of Quenchbodies using antibody-binding proteins, Analytical Methods, The Royal Society of Chemistry, vol. 8, p. 7774-7779, Oct. 2016.
• Hiroyuki Ohashi, Takashi Matsumoto, Hee-Jin Jeong, Jinhua Dong, Ryoji Abe, Hiroshi Ueda. Insight into the working mechanism of Quenchbody: Transition of the dye around antibody variable region that fluoresces upon antigen binding, Bioconjugate Chemistry, American Chemical Society, vol. 27, no. 10, p. 2248-2253, Sept. 2016.
• Chan-I Chung, Ryoji Makino, Yuki Ohmuro, Hiroshi Ueda. Development of a fluorescent protein-antibody FRET probe for the detection and imaging of osteocalcin, J. BIOSCI. BIOENG., vol. 123, no. 2, p. 272-276, Sept. 2016.
• 上田 宏, 董 金華, Hee-Jin Jeong, 阿部 亮二. ネオバイオ分子としてのQ-body, 生物工学会誌, vol. 94, no. 8, p. 488-91, Aug. 2016.
• Y. Ohmuro, H. Ueda. A protein-protein interaction assay FlimPIA based on the functional complementation of mutant firefly luciferases, Methods Mol. Biol., Vol. 1461, p. 131-142, Aug. 2016.
• J. Gholami, M. Manteghian, A. Badiei, H. Ueda, M. Javanbakht. N-butylamine functionalized graphene oxide for detection of Iron(III) by photoluminescence quenching, Luminescence, vol. 31, p. 229-235, May 2016.  公式リンク  
  
• Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, Hiroshi Ueda. Creation of a ligand-dependent enzyme by fusing circularly permuted antibody variable region domains, BIOCONJUGATE CHEM, vol. 27, p. 868-873, Feb. 2016.  公式リンク  
  
• Renee Dale, Yuki Ohmuro-Matsuyama, Hiroshi Ueda, Naohiro Kato. Mathematical model of the firefly luciferase complementation assay reveals a non-linear relationship between the detected luminescence and the affinity of the protein pair being analyzed, PLOS ONE, vol. 11, Feb. 2016.  公式リンク  
  
• Yuki Omuro, Mori, K., Hamada, H., Hiroshi Ueda, Yamaji, H.. Electrostatic engineering of the interface between heavy and light chains promotes antibody Fab fragment production., CYTOTECHNOLOGY, Feb. 2016.
• Jinhua Dong, Hee-Jin Jeong, Hiroshi Ueda. Preparation of Quenchbodies by protein transamination reaction, J BIOSCI BIOENG, vol. 122, no. 1, p. 125-130, Jan. 2016.  公式リンク  
  
• Hee-Jin Jeong, Takuya Kawamura, Jinhua Dong, Hiroshi Ueda. Qーbodies from recombinant single-chain Fv fragment with better yield and expanded palette of fluorophores, ACS Sensors, vol. 1, no. 1, p. 88-94, Oct. 2015.
• M. Kurihara, Y. Ohmuro-Matsuyama, K. Ayabe, T. Yamashita, H. Yamaji, H. Ueda. Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions, Biotechnol. J., Vol. 11, No. 1, p. 91-99, Sept. 2015.
• M. Komatsu, J. Dong, H. Ueda, R. Arai. Crystal structure of Fab fragment of an anti-osteocalcin C-terminal peptide antibody KTM219, Photon Factory Act. Rep., Vol. 2014, Part B, p. 205, Aug. 2015.  公式リンク  
  
• J. Gholami, M. Manteghian, A. Badiei, M. Javanbakht, H. Ueda. Label free detection of vitamin B12 based on fluorescence quenching of graphene oxide nanolayer, Fullerenes, Nanotubes and Carbon Nanostructures, vol. 23, no. 10, p. 878-884, May 2015.  公式リンク  
  
• Jinhua Dong, Tomoki Kojima, Hiroyuki Ohashi, Hiroshi Ueda. Optimal fusion of antibody binding domains resulted in higher affinity and wider specificity, J BIOSCI BIOENG, Vol. 120, No. 5, p. 504-509, Apr. 2015.
• H.J. Jeong, S. Itayama, H. Ueda. A signal-on fluorosensor based on quench-release principle for sensitive detection of antibiotic rapamycin, Biosensors, MDPI AG, Vol. 5, No. 2, p. 131-140, Mar. 2015.  公式リンク  
  
• C.I. Chung, R. Makino, J. Dong, H. Ueda. Open flower fluoroimmunoassay: a general method to make fluorescent protein-based immunosensor probes, ANAL CHEM, ACS, Vol. 87, p. 3513-9, Feb. 2015.
• K. Hayashi, Y. Tomozoe, K. Nagai, K. Matsuba, M. Mitsumori, Y. Hiraishi, T. Matsumura, 上田 宏, 神谷 典穂. イムノアッセイを目的とした融合タンパク質試薬の設計と生産プロセスの最適化, 化学工学会, Vol. 41, No. 1, p. 38-42, Jan. 2015.  公式リンク  
  
• H. Veisi, J. Gholami, H. Ueda, P. Mohammadi, M. Noroozi. Magnetically palladium catalyst stabilized by diaminoglyoxime-functionalized magnetic Fe3O4 nanoparticles as active and reusable catalyst for Suzuki coupling reactions, J. Mol. Catal. A: Chemical, Elsevier, Vol. 396, p. 216-223, Oct. 2014.
• Hee-Jin Jeong, Hiroshi Ueda. Strategy for Making a Superior Quenchbody to Proteins: Effect of the Fluorophore Position, Sensors (Basel, Switzerland), MDPI, vol. 14, p. 13285-13297, July 2014.  公式リンク  
  
• Yoshiyuki Sasajima, Yusuke Kohama, Miki Kojima-Misaizu, Naoya Kurokawa, Yuko Hara, Jinhua Dong, Masaki Ihara, Hiroshi Ueda. Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases, Molecular Biotechnology, Springer, vol. 56, no. 10, p. 953-961, June 2014.  公式リンク  
  
• R. Abe, Hee-Jin Jeong, D. Arakawa, J.H. Dong, Hiroyuki Ohashi, R. Kaigome, F. Saiki, Kyosuke Yamane, Hiroaki Takagi, H.Ueda. Ultra Q-bodies : quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence, Scientific Reports, Nature Publishing Group, vol. 4, p. 4640, Apr. 2014.  公式リンク  
  
• Hiroshi Ueda, Yuko Hara, Yuki Ohmuro. Improved protein-protein interaction assay FlimPIA by the entrapment of luciferase conformation, Anal. Chem., Vol. 86, p. 2013-2018, Dec. 2013.  公式リンク  
  
• Sharif Hasan, Jinhua Dong, Yuko Hara, Yoshihito Morizane, Futoshi Shibasaki, Hiroshi Ueda. Protein-based open sandwich immuno-PCR for sensitive detection of small biomarkers, Anal. Sci., Vol. 29, pp. 871-876, Sept. 2013.
• Yuki Ohmuro-Matsuyama, Kota Nakano, Aoi Kimura, Keiichi Ayabe, Masaki Ihara, Takeshi Wada, Yuki Ohmuro-Matsuyama, Kota Nakano, Aoi Kimura, Keiichi Ayabe, Masaki Ihara, Takeshi Wada, Hiroshi Ueda. A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases, Anal. Chem., Vol. 85, pp. 7935-7940, Aug. 2013.
• Yuko Hara, Jinhua Dong, Hiroshi Ueda. Open-sandwich immunoassay for sensitive and broad-range detection of a shellfish toxin gonyautoxin, Anal Chim Acta, Vol. 793, pp. 107-113, June 2013.
• Jinhua Dong, Akira Sakurai, Namiko Nomura, Enoch Y. Park, Futoshi Shibasaki, Hiroshi Ueda. Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus, PLOS ONE, Vol. 8, No. 4, pp. e61158, Apr. 2013.
• Yuki Ohmuro-Matsuyama, Chan-I Chung, Hiroshi Ueda. Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments, BMC Biotechnol., Vol. 13, pp. 31, Mar. 2013.
• Chungyong Han, Masaki Ihara, Hiroshi Ueda. Expression of an antibody-enzyme complex by the L-chain fusion method, J. Biosci. Bioeng., Vol. 116, No. 1, pp. 17-21, Feb. 2013.
• Hee-Jin Jeong, Yuki Ohmuro-Matsuyama, Hiroyuki Ohashi, Fuyuki Ohsawa, Yoshiro Tatsu, Masaki Inagaki, Hiroshi Ueda. Detection of vimentin serine phosphorylation by multicolor Quenchbodies, Biosensors 2012, Biosens. Bioelectron., Elsevier, Vol. 40, pp. 17-23, 2013.
• Yuichiro Tone, Masahiro Kawahara, Daichi Kawaguchi, Hiroshi Ueda, Teruyuki Nagamune. Death signalobody: Inducing conditional cell death in response to a specific antigen, Hum. Gen. Ther. Methods, Vol. 24, pp. 141-150, 2013.
• Xiaobo Liu, Michael Eichenberger, Yuuichiro Fujioka, Jinhua Dong, Hiroshi Ueda. Improved detection sensitivity and selectivity attained by Open-Sandwich selection of an anti-estradiol antibody, Anal. Sci., Vol. 28, No. 9, pp. 861-867, Sept. 2012.
• Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, Hiroshi Ueda. Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments, Bioconj. Chem., Vol. 23, No. 7, pp. 1463-1469, June 2012.
• Koichiro Saka, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. Activation of target signal transducers utilizing chimeric receptors with signaling-molecule binding motifs, Biotechnol. Bioeng., Vol. 109, pp. 1528-1537, Jan. 2012.
• Etsuji Kaneko, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. Growth Control of Genetically Modified Cells via Antibody/c-Kit Chimera, J. Biosci. Bioeng., Vol. 113, pp. 641-646, Jan. 2012.
• Jinhua Dong, Sharif Hasan, Yuuichiro Fujioka, Hiroshi Ueda. Detection of small molecule diagnostic markers with phage-based Open-Sandwich Immuno-PCR, J. Immunol. Methods, Vol. 377, pp. 1-7, Jan. 2012.
• Ryoji Abe, Hiroyuki Ohashi, Issei Iijima, Masaki Ihara, Hiroaki Takagi, Takahiro Hohsaka, Hiroshi Ueda. “Quenchbodies” : quench-based antibody probes that show antigen-dependent fluorescence, J. Am. Chem. Soc., Vol. 133, No. 43, pp. 17386-94, Oct. 2011.
• Miki Kojima, Hiroto Iwai, Jinhua Dong, Shean-Lee Lim, Shigekazu Ito, Koichi Okumura, Masaki Ihara, Hiroshi Ueda. Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules, Bioconj. Chem., Vol. 22, No. 4, pp. 633-641, Mar. 2011.
• Kamrun Nahar Islam, Masaki Ihara, Jinhua Dong, Noriyuki Kasagi, Toshihiro Mori, Hiroshi Ueda. Direct construction of an open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4, Anal. Chem., Vol. 83, pp. 1008-1014, Jan. 2011.
• Masahiro Kawahara, Jianhong Chen, Takahiro Sogo, Jinying Teng, Makoto Otsu, Masafumi Onodera, Hiromitsu Nakauchi, Hiroshi Ueda, Teruyuki Nagamune. Growth promotion of genetically modified hematopoietic progenitors using an antibody/c-Mpl chimera, Cytokine, Vol. 55, pp. 402-408, 2011.
• Hiroto Iwai, Bengü Oztürk, Masaki Ihara, Hiroshi Ueda. Antibody affinity maturation in vitro using unconjugated peptide antigen, Protein Eng. Des. Sel., Vol. 23, No. 4, pp. 185-193, Feb. 2010.
• Takashi Dojima, Takuya Nishina, Tatsuya Kato, Hiroshi Ueda, Enoch Y. Park. Production and purification of baculovirus surface displaying scFv in Bombyx mori larvae using BmNPV bacmid, Biotechnol. Appl. Biochem., Vol. 57, No. 2, pp. 63-69, 2010.
• Yoshiyuki Sasajima, Ryohei Iwasaki, Kouhei Tsumoto, Izumi Kumagai, Masaki Ihara, Hiroshi Ueda. Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells, J. Immunol. Methods, Vol. 361, pp. 57-63, 2010.
• Yoshiyuki Ohiro, Hiroshi Ueda, Norio Shibata, Teruyuki Nagamune. Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology, J. Biosci. Bioeng., Vol. 109, No. 1, pp. 15-19, 2010.
• Kamrun Nahar Islam, Masaki Ihara, Jinhua Dong, Noriyuki Kasagi, Toshihiro Mori, Hiroshi Ueda. Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples, Bioanalysis, Vol. 2, No. 10, pp. 1683-1687, 2010.
• Masaki Ihara, Amane Yoshikawa, Yushu Wu, Hiroko Takahashi, Kazuma Mawatari, Kiyohito Shimura, Kae Sato, Takehiko Kitamori, Hiroshi Ueda. Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device, Lab. Chip, Vol. 10, pp. 92-100, 2010.
• Takashi Dojima, Takuya Nishina, Tatsuya Kato, Hirokazu Yagi, Koichi Kato, Hiroshi Ueda, Enoch Y. Park. Improved secretion of molecular chaperone-assisted human IgG in silkworm, and no alterations in their N-Linked glycan structures, Biotechnol. Prog., Vol. 26, No. 1, pp. 232-238, 2010.
• Hiroshi Iwanaga, Hirohiko Tsuzuki, Youichirou Kamiyama, Hiroshi Ueda. Verification of specific reaction between airborne antigen and immobilized antibody at gas-solid interface, Anal. Sci., Vol. 25, No. 9, pp. 1101-1106, Sept. 2009.
• Kento Tanaka, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera, Biotechnol. Prog., Vol. 25, pp. 1138-1145, 2009.
• Takashi Dojima, Takuya Nishina, Tatsuya Kato, Hiroshi Ueda, Enoch Y. Park. Comparison of the efficiencies of different affinity tags in the purification of a recombinant secretory protein expressed in silkworm larval hemolymph, Biotechnol. Bioproc. Eng., Vol. 14, pp. 281-287, 2009.
• Junhua Dong, Masaki Ihara, Hiroshi Ueda. Antibody Fab display system that can perform open sandwich ELISA, Anal. Biochem., Vol. 386, pp. 36-44, 2009.
• Masaki Ihara, Tatsuya Suzuki, Norihiro Kobayashi, Jun-ichi Goto, Hiroshi Ueda. Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol, Anal. Chem., Vol. 81, pp. 8298-8304, 2009.
• Motoki Ishikiriyama, Takuya Nishina, Tatsuya Kato, Hiroshi Ueda, Enoch Y. Park. Human Single-chain Antibody Expression in the Hemolymph and Fat Body of Silkworm Larvae and Pupae Using BmNPV Bacmids, J. Biosci. Bioeng., Vol. 107, No. 1, pp. 67-72, 2009.
• Ryohei Iwasaki, Hiroki Kiuchi, Masaki Ihara, Toshihiro Mori, Masayuki Kawakami, Hiroshi Ueda. Trans-splicing as a novel method to rapidly produce antibody fusion proteins, Biochem. Biophys. Res. Commun., Vol. 384, No. 3, pp. 316-321, 2009.
• Wenhai Liu, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. The influence of domain structures on the signal transduction of chimeric receptors derived from the erythropoietin receptor, J. Biochem., Vol. 145, No. 5, pp. 575-584, 2009.
• Enoch Y. Park, Motoki Ishikiriyama, Takuya Nishina, Tatsuya Kato, Hirokazu Yagi, Koichi Kato, Hiroshi Ueda. Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure, J. Biotechnol., Vol. 139, pp. 108-114, 2009.
• Toshiya Sakata, Masaki Ihara, Izumi Makino, Yuji Miyahara, Hiroshi Ueda. Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen, Anal. Chem., Vol. 81, No. 18, pp. 7532–7537, 2009.
• Takahiro Sogo, Masahiro Kawahara, Hiroshi Ueda, Makoto Otsu, Masafumi Onodera, Hiromitsu Nakauchi, Teruyuki Nagamune. T cell growth control using hapten-specific antibody/interleukin-2 receptor chimera, Cytokine, Vol. 46, pp. 127-136, 2009.
• Tamotsu Zako, Hiroyasu Nagata, Naofumi Terada, Arata Utsumi, Masafumi Sakono, Masafumi Yohda, Hiroshi Ueda, Kohei Soga, Mizuo Maeda. Cyclic RGD Peptide-Labeled Upconversion Nanophosphors for Tumor Cell-Targeted Imaging, Biochem. Biophys. Res. Commun., Vol. 381, pp. 54-58, 2009.
• S Takeda, S Tsukiji, H Ueda, T Nagamune. Covalent split protein fragment-DNA hybrids generated through N-terminus-specific modification of proteins by oligonucleotides, Org. Biomol. Chem., Vol. 6, No. 12, pp. 2187-2194, 2008.
• Masafumi Sakono, Tamotsu Zako, Hiroshi Ueda, Masafumi Yohda, Mizuo Maeda. Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin, FEBS J., Vol. 275, No. 23, pp. 5982-5993, 2008.
• Wenhai Liu, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. Construction of a fluorescein-responsive chimeric receptor with strict ligand dependency, Biotechnol. Bioeng., Vol. 101, pp. 975-984, 2008.
• Younggiu Lee, Tadao Asami, Isomaro Yamaguchi, Hiroshi Ueda, Yoshihito Suzuki. A new gibberellin detection system in living cells based on VH/VL interaction of antibody, Biochem. Biophys. Res. Commun., Vol. 376, pp. 134-138, 2008.
• Masahiro Kawahara, Yusuke Shimo, Takahiro Sogo, Azusa Hitomi, Hiroshi Ueda, Teruyuki Nagamune. Antigen-mediated migration of murine pro-B Ba/F3 cells via an antibody/receptor chimera, J. Biotechnol., Vol. 133, pp. 154–161, 2008.  公式リンク  
  
• Kazutoshi Yoshitake, Shoko Waki, Hiroshi Ueda. Dimerization-based homogeneous fluorosensor proteins for the detection of specific dsDNA, Biosens. Bioelectron., Vol. 23, No. 8, pp. 1266-1271, 2008.
• Takahiro Sogo, Masahiro Kawahara, Kouhei Tsumoto, Izumi Kumagai, Hiroshi Ueda, Teruyuki Nagamune. Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera, J. Immunol. Methods, Vol. 337, pp. 16-23, 2008.
• Y. Ohiro, H. Ueda, N. Shibata, T. Nagamune. Enhanced fluorescence resonance energy transfer immunoassay with improved sensitivity based on the Fab'-based immunoconjugates, Anal. Biochem., Vol. 360, No. 2, pp. 266-272, 2007.
• Tatsuya Suzuki, Yuriko Munakata, Kazuki Morita, Tatsuya Shinoda, Hiroshi Ueda. Sensitive detection of estrogenic mycotoxin zearalenone by open sandwich immunoassay, Anal. Sci., Vol. 23, pp. 65-70, 2007.
• Masahiro Kawahara, Toshiaki Inoue, Xianying Ren, Takahiro Sogo, Hidetoshi Yamada, Motonobu Katoh, Hiroshi Ueda, Mitsuo Oshimura, Teruyuki Nagamune. Antigen-mediated growth control of hybridoma cells via a human artificial chromosome, Biochim. Biophys. Acta, Vol. 1770, No. 2, pp. 206-212, 2007.
• Masahiro Kawahara, Hiroshi Ueda, Kouhei Tsumoto, Izumi Kumagai, Teruyuki Nagamune. Mimicry of erythropoietin and interleukin-6 signaling by an antibody/cytokine receptor chimera in murine myeloid 32D cells, J. Biochem., Vol. 141, pp. 563-571, 2007.
• Shean-Lee Lim, Hiroko Ichinose, Tatsuya Shinoda, Hiroshi Ueda. Noncompetitive detection of low molecular weight peptides by open sandwich immunoassay, Anal. Chem., Vol. 79, No. 16, pp. 6193-6200, 2007.
• Yoshiaki Sugimura, Hiroshi Ueda, Masatoshi Maki, Kiyotaka Hitomi. Novel site-specific immobilization of functional protein using a preferred substrate sequence for transglutaminase 2, J. Biotechnol., Vol. 131, No. 2, pp. 121-127, 2007.
• Masahiro Kawahara, Yuko Ogo, Kouhei Tsumoto, Izumi Kumagai, Hiroshi Ueda, Teruyuki Nagamune. Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer, Cytotechnology, Vol. 52, pp. 171-179, 2006.
• Yoshiyuki Sasajima, Takahide Aburatani, Kenzo Sakamoto, Hiroshi Ueda. Detection of protein tyrosine phosphorylation by Open Sandwich fluoroimmunoassay, Biotechnol. Prog., Vol. 22, pp. 968-973, 2006.
• Hidetoshi Yamada, Atsushi Kunisato, Masahiro Kawahara, Candice G. T. Tahimic, Xianying Ren, Hiroshi Ueda, Teruyuki Nagamune, Motonobu Katoh, Toshiaki Inoue, Mitsuo Nishikawa, Mitsuo Oshimura. Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome, J. Hum. Genet., Vol. 51, pp. 147-150, 2006.
• Kenji Masuda, Kenzo Sakamoto, Miki Kojima, Takahide Aburatani, Takuya Ueda, Hiroshi Ueda. The role of interface framework residues in determining antibody VH/VL interaction strength and antigen binding affinity, FEBS J., Vol. 273, pp. 2184-2194, 2006.
• Miki Kojima, Keiichi Ayabe, Hiroshi Ueda. Importance of terminal residues on circularly permutated Escherichia coli alkaline phosphatase with high specific activity, J. Biosci. Bioeng., Vol. 100, No. 2, pp. 197-202, 2005.
• Keiichi Ayabe, Tamotsu Zako, Hiroshi Ueda. The role of firefly luciferase C-terminal domain in efficient coupling of adenylation and oxidative steps, FEBS Lett., Vol. 579, No. 20, pp. 4389-4394, 2005.
• M. Kawahara, Y. Ogo, H. Ueda, T. Nagamune. Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain, Protein Eng. Des. Sel., Vol. 17, No. 10, pp. 715-719, 2004.
• M. Kawahara, H. Ueda, K. Tsumoto, I. Kumagai, T. Nagamune. AMEGA: Antigen-MEdiated Genetically modified cell Amplification, J. Immunol. Methods, Vol. 284, No. 1-2, pp. 187-194, 2004.
• Naoto Komiya, Hiroshi Ueda, Yoshiyuki Ohiro, Teruyuki Nagamune. Homogeneous sandwich immunoassay based on the enzymatic complementation induced by single-chain Fv fragments, Anal. Biochem., Vol. 327, pp. 241-246, 2004.
• Mina Okochi, Tomoko Nomura, Tamotsu Zako, Ryo Iizuka, Hiroshi Ueda, Takashi Funatsu, Michel Leroux, Masafumi Yohda. Kinetics and binding sites for interaction of prefoldin with group II chaperonin: contiguous non-native substrate and chaperonin binding sites in archaeal prefoldin, J. Biol. Chem., Vol. 279, No. 30, pp. 31788-31795, 2004.
• Paivi Pihkala, Masahiro Kawahara, Hiroshi Ueda, Teruyuki Nagamune. An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv, Biochem. Biophys. Res. Commun., Vol. 324, pp. 1165-1172, 2004.
• Takeshi Takazawa, Noriho Kamiya, Hiroshi Ueda, Teruyuki Nagamune. Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase, Biotechnol. Bioeng., Vol. 86, No. 4, pp. 399-404, 2004.  公式リンク  
  
• H. Ueda, P. Kristensen, G. Winter. Stabilization of antibody VH-domains by proteolytic selection, J. Mol. Catal. B:Enzymatic, Vol. 28, No. 4-6, pp. 173-180, 2004.
• Bei-Wen Ying, Hideki Taguchi, Hiroshi Ueda, Takuya Ueda. Chaperone-assisted folding of a single-chain antibody in a reconstituted translation system, Biochem. Biophys. Res. Commun., Vol. 320, pp. 1359-1364, 2004.
• Masahiro Kawahara, Shinya Ishii, Kouhei Tsumoto, Izumi Kumagai, Hiroshi Ueda, Teruyuki Nagamune. Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily, Biochem. Pharmacol., Vol. 68, No. 3, pp. 539-548, 2004.
• Etsuji Kaneko, Masahiro Kawahara, Kouhei Tsumoto, Izumi Kumagai, Hiroshi Ueda, Teruyuki Nagamune. Antigen-mediated genetically modified cell amplification (AMEGA) with a single vector transduction, J. Chem. Eng. Jpn., Vol. 37, pp. 1259-1264, 2004.
• Masahiro Kawahara, Hiroko Kimura, Hiroshi Ueda, Teruyuki Nagamune. Selection of genetically modified cell population using hapten-specific antibody/receptor chimera, Biochem. Biophys. Res. Commun., Vol. 315, pp. 132-138, 2004.
• Takahide Aburatani, Kenzo Sakamoto, Kenji Masuda, Kosuke Nishi, Hideo Ohkawa, Teruyuki Nagamune, Hiroshi Ueda. A general method to select antibody fragments suitable for noncompetitive detection of monovalent antigens, Anal. Chem., Vol. 75, No. 16, pp. 4057-4064, 2003.
• Noriho Kamiya, Takeshi Takazawa, Tsutomu Tanaka, Hiroshi Ueda, Teruyuki Nagamune. Site-specific crosslinking of functional proteins by transglutamination, Enzyme Microb. Tech., Vol. 33, pp. 492-496, 2003.
• Masahiro Kawahara, Hiroshi Ueda, Sumiyo Morita, Kouhei Tsumoto, Izumi Kumagai, Teruyuki Nagamune. Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch, Nucleic Acids Res., Vol. 31, No. 7, pp. e32, 2003.
• Atsushi Kitayama, Hiroaki Yoshizaki, Yoshihiro Ohmiya, Hiroshi Ueda, Teruyuki Nagamune. Creation of a thermostable firefly luciferase with pH-insensitive luminescent color, Photochem. Photobiol., Vol. 77, No. 3, pp. 333-338, 2003.
• Hiroshi Ueda, Tomoichi Yokozeki, Ryoichi Arai, Kouhei Tsumoto, Izumi Kumagai, Teruyuki Nagamune. An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation, J. Immunol. Methods, Vol. 279, No. 1-2, pp. 209-218, 2003.
• Tamotsu Zako, Keiichi Ayabe, Takahide Aburatani, Noriho Kamiya, Atsushi Kitayama, Hiroshi Ueda, Teruyuki Nagamune. Luminescent and substrate binding activities of firefly luciferase N-terminal domain, Biochim. Biophys. Acta, Vol. 1649, pp. 183-189, 2003.
• Takahide Aburatani, Hiroshi Ueda, Teruyuki Nagamune. Importance of a CDR H3 basal residue in VH/VL interaction of human antibodies, J. Biochem., Vol. 132, No. 5, pp. 775-782, 2002.
• Tomoichi Yokozeki, Hiroshi Ueda, Ryoichi Arai, Walt Mahoney, Teruyuki Nagamune. A homogeneous noncompetitive immunoassay for the detection of small haptens, Anal. Chem., Vol. 74, No. 11, pp. 2500-2504, 2002.
• Yukari Yamakawa, Hiroshi Ueda, Atsushi Kitayama, Teruyuki Nagamune. Rapid homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase, J. Biosci. Bioeng., Vol. 93, No. 6, pp. 537-542, 2002.
• Yoshiyuki Ohiro, Ryoichi Arai, Hiroshi Ueda, Teruyuki Nagamune. A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction, Anal. Chem., Vol. 74, No. 22, pp. 5786-5792, 2002.
• M. Kawahara, H. Ueda, K. Tsumoto, I. Kumagai, W. Mahoney, T. Nagamune. Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera, J. Biosci. Bioeng., Vol. 93, No. 4, pp. 399-404, 2002.
• Ryoichi Arai, Hideyuki Nakagawa, Atsushi Kitayama, Hiroshi Ueda, Teruyuki Nagamune. Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein, J. Biosci. Bioeng., Vol. 94, No. 4, pp. 362-364, 2002.
• Ryoichi Arai, Hideyuki Nakagawa, Kouhei Tsumoto, Walt Mahoney, Izumi Kumagai, Hiroshi Ueda, Teruyuki Nagamune. Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer, Anal. Biochem., Vol. 289, No. 1, pp. 77-81, 2001.  公式リンク  
  
• Tamotsu Zako, K. Harada, T. Mannen, S. Yamaguchi, A. Kitayama, Hiroshi Ueda, Teruyuki Nagamune. Monitoring the refolding process of immobilized firefly luciferase using a biosensor based on surface plasmon resonance, J. Biochem., Vol. 129, No. 1, pp. 1-4, 2001.
• Seiji Ura, Hiroshi Ueda, Jun Kazami, Genji Kawano, Teruyuki Nagamune. Single cell reporter assay using cell surface displayed Vargula luciferase, J. Biosci. Bioeng., Vol. 92, No. 6, pp. 575-579, 2001.
• Masahiro Kawahara, Hiroshi Ueda, Kouhei Tsumoto, Izumi Kumagai, Walt Mahoney, Teruyuki Nagamune. A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer, J. Biochem., Vol. 130, No. 2, pp. 305-312, 2001.
• Ryoichi Arai, Hiroshi Ueda, Atsushi Kitayama, Noriho Kamiya, Teruyuki Nagamune. Design of the linkers which effectively separate domains of a bifunctional fusion protein, Protein Eng., Vol. 14, No. 8, pp. 529-532, 2001.
• Masahiro Kawahara, Akito Natsume, Satoshi Terada, Koichi Kato, Kouhei Tsumoto, Izumi Kumagai, Masao Miki, Walt Mahoney, Hiroshi Ueda, Teruyuki Nagamune. Replacing factor dependency with that for lysozyme: Affordable culture of IL-6 dependent hybridoma by transfecting artificial cell surface receptor, Biotechnol. Bioeng., Vol. 74, No. 5, pp. 416-423, 2001.
• Ryoichi Arai, Hiroshi Ueda, Kouhei Tsumoto, Walt C. Mahoney, Izumi Kumagai, Teruyuki Nagamune. Fluorolabeling of antibody variable domains with green fluorescent protein variants: Application to an energy transfer-based homogeneous immunoassay, Protein Eng., Vol. 13, No. 5, pp. 369-376, 2000.  公式リンク  
  
• Chikako Suzuki, Hiroshi Ueda, Walt Mahoney, Teruyuki Nagamune. Open Sandwich Enzyme-Linked Immunosorbent Assay for the quantitation of small haptens, Anal. Biochem., Vol. 286, No. 2, pp. 238-246, 2000.  公式リンク  
  
• Hiroshi Ueda, Masahiro Kawahara, Takahide Aburatani, Kouhei Tsumoto, Kazuo Todokoro, Eiji Suzuki, Hajime Nishimura, Paula A. Schueler, Greg Winter, Walt C. Mahoney, Izumi Kumagai, Teruyuki Nagamune. Cell-growth control by monomeric antigen; the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor, J. Immunol. Methods, Vol. 241, No. 1-2, pp. 159-170, 2000.  公式リンク  
  
• T. Zako, H. Deguchi, A. Kitayama, H. Ueda, T. Nagamune. Refolding of firefly luciferase immobilized on agarose beads, J. Biochem., Vol. 127, No. 3, pp. 351-354, 2000.
• Hiroshi Ueda, Kazuishi Kubota, Yu Wang, Kouhei Tsumoto, Walt Mahoney, Izumi Kumagai, Teruyuki Nagamune. Homogeneous non-competitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (Open sandwich FIA), Biotechniques, Vol. 27, pp. 738-742, 1999.
• Chikako Suzuki, Hiroshi Ueda, Kouhei Tsumoto, Walt Mahoney, Izumi Kumagai, Teruyuki Nagamune. Open Sandwich ELISA with VH- / VL- alkaline phosphatase fusion proteins, J. Immunol. Methods, Vol. 224, No. 1-2, pp. 171-184, 1999.  公式リンク  
  
• Rhoichi Arai, Hiroshi Ueda, Teruyuki Nagamune. Construction of a chimeric protein between protein G and fluorescence-enhanced green fluorescent protein, and its application to immunoassay, J. Ferment. Bioeng., Vol. 86, No. 5, pp. 440-445, 1998.
• 上田 宏, 喜多山 篤, 鈴木 栄二, 長棟 輝行. カテコール2,3-ジオキシゲナーゼ−プロテインＧキメラ蛋白質の作製とその免疫測定への応用, 化学工学論文集, Vol. 24, No. 2, pp. 1-5, 1998.
• Yon H. Kim, Takehiro Iida, Tetsuo Fujita, Satoshi Terada, Atsushi Kitayama, Hiroshi Ueda, Edward V. Prochownik, Eiji Suzuki. Establishment of an apoptosis-resistant and growth-controllable cell line by transfecting with inducible antisense c-jun gene, Biotechnol. Bioeng., Vol. 58, No. 1, pp. 65-72, 1998.  公式リンク  
  
• Eiko Murayama-Kayano, Sadaaki Yoshimatsu, Toshiaki Kayano, Takeshi Nishio, Hiroshi Ueda, Teruyuki Nagamune. Application of the random amplified polymorphic DNA (RAPD) technique to distinguish species of the red tide phytoplankton Chattonella (Raaphydophyceae), J. Ferment. Bioeng., Vol. 85, No. 3, pp. 343-345, 1998.
• 深野 一, 上田 宏, 鈴木 栄二. 競合PCR法における増幅過程の数値モデル, 生物工学会誌, Vol. 75, No. 1, pp. 1-8, 1997.
• Kouhei Tsumoto, Yoshiyuki Nishimiya, Nobuhiro Kasai, Hiroshi Ueda, Teruyuki Nagamune, Kyoko Ogasahara, Katsuhide Yutani, Kenji Tokuhisa, Masaaki Matsushima, Izumi Kumagai. Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen, Protein Eng, Vol. 10, pp. 1311-1318, 1997.
• S. Terada, Y. Itoh, H. Ueda, E. Suzuki. Characterization and fed-batch culture of hybridoma overexpressing apoptosis supressing gene bcl-2, Cytotechnology, Vol. 24, pp. 135-141, 1997.
• E. Suzuki, S. Terada, H. Ueda, T. Fujita, T. Komatsu, S. Takayama, J. C. Reed. Establishing appoptosis resistant cell lines for improving protein productivity, Cytotechnology, Vol. 23, pp. 55-59, 1997.
• C. Suzuki, H. Ueda, E. Suzuki, T. Nagamune. Construction and characterization of an anti-hapten ScFv-alkaline phosphatase chimeric protein, J. Biochem., Vol. 122, No. 2, pp. 322-329, 1997.
• Yumi Maeda, Hiroshi Ueda, Jun Kazami, Genji Kawano, Eiji Suzuki, Teruyuki Nagamune. Engineering of functional chimeric protein G-Vargula luciferase, Anal. Biochem., Vol. 249, pp. 147-152, 1997.
• Tetsuo Fujita, Satoshi Terada, Katsuyuki Fukuoka, Atsushi Kitayama, Hiroshi Ueda, Eiji Suzuki. Reinforcing apoptosis-resistance of COS and myeloma cells by transfecting with bcl-2 gene, Cytotechnology, Vol. 25, pp. 25-33, 1997.
• 高橋 一成, 上田 宏, 鈴木 栄二. 動物細胞潅流培養の温度特性, 化学工学論文集, Vol. 22, No. 2, pp. 404-407, 1996.
• H. Ueda, K. Tsumoto, K. Kubota, E. Suzuki, T. Nagamune, H. Nishimura, P. A. Schueler, G. Winter, I. Kumagai, W. C. Mahoney. Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region, Nat. Biotechnol., Vol. 14, No. 12, pp. 1714-1718, 1996.
• S. Terada, E. Suzuki, H. Ueda, F. Makishima. Cytokines involving gp130 in signal transduction suppressed growth of a mouse hybridoma cell line and enhanced its antibody production, Cytokine, Vol. 8, No. 12, pp. 889-894, 1996.
• T. Shindo, H. Ueda, F. Makishima, H. Nishimura, E. Suzuki. Method for obtaining mutants defective in immunoglobulin m transcription factors, Biosci. Biotech. Biochem., Vol. 60, No. 11, pp. 1837-1840, 1996.
• Yumi Maeda, Hiroshi Ueda, Jun Kazami, Genji Kawano, Eiji Suzuki, Teruyuki Nagamune. Truncation of Vargula luciferase still resulted in retention of luminescent activity, J. Biochem., Vol. 119, No. 4, pp. 601-603, 1996.
• Y. Maeda, H. Ueda, T. Hara, J. Kazami, G. Kawano, E. Suzuki, T. Nagamune. Expression of a bifunctional chimeric protein A-Vargula luciferase in mammalian cells, Biotechniques, Vol. 20, No. 1, pp. 116-121, 1996.
• T. Fujita, S. Terada, H. Ueda, E. Suzuki. Overexpression of Bcl-2 improved survival of COS-1 cells and enhanced transient protein production, J. Ferment. Bioeng., Vol. 82, No. 6, pp. 589-591, 1996.
• A. Kitayama, T. Achioku, T. Yanagawa, K. Kanou, M. Kikuchi, H. Ueda, E. Suzuki, H. Nishimura, T. Nagamune, Y. Kawakami. Cloning and characterization of extradiol aromatic ring-cleavage dioxygenases of Pseudomonas aeruginosa JI104, J. Ferment. Bioeng., Vol. 82, No. 3, pp. 217-223, 1996.
• Y. Kohara, H. Ueda, E. Suzuki. Enhanced settling of mammalian cells in tanks with inclined plates / simulation by fluid mechanical model and experiment, J. Chem. Eng. Japan, Vol. 28, No. 6, pp. 703-707, 1995.
• Hiroshi Ueda, Masaki Nakanishi, Eiji Suzuki, Teruyuki Nagamune. Enhancement of mutation frequency with nucleotide triphosphate analogs in PCR random mutagenesis, J. Ferment. Bioeng., Vol. 79, No. 3, pp. 303-305, 1995.
• T. Shindo, H. Ueda, E. Suzuki, H. Nishimura. A catechol 2,3-dioxygenase gene as a reporter, Biosci. Biotech. Biochem., Vol. 59, No. 2, pp. 314-315, 1995.
• Y. Itoh, H. Ueda, E. Suzuki. Over-expression of bcl-2, apoptosis supressing gene, prolonged viable culture period of hybridoma and enhanced antibody production, Biotech. Bioeng., Vol. 48, pp. 118-122, 1995.
• K. Takahashi, S. Terada, H. Ueda, F. Makishima, E. Suzuki. Growth-rate supression of cultured mammalian cells enhances protein productivity, Cytotechnol., Vol. 15, pp. 57-64, 1994.
• H. Ueda, M. Kikuchi, S. Yagi, H. Nishimura. Antigen responsive antibody-receptor kinase chimera, Bio/Technol., Vol. 10, No. 4, pp. 430-433, 1992.
• T. Shindo, H. Ueda, F. Makishima, E. Suzuki, H. Nishimura. Efficient selection of µm-mutants from µm-expressing myeloma cells by treatment with ricin A-conjugated anti-µ antibody, Somatic Cell and Molecular Genetics, Vol. 18, No. 6, pp. 553-558, 1992.
• Ueda, H., M. Kikuchi, A. Hasegawa, S. Yagi, H. Nishimura. 新しい免疫センサーのためのIgM-上皮増殖因子受容体融合タンパク質の試作とそのミエローマ細胞における膜発現, 化学工学論文集, Vol. 17, No. 3, pp. 547-552, 1991.

著書

• Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi. Development of a single fluorescent protein-based green glucose indicator by semirational molecular design and molecular evolution., Methods Mol Biol, Springer, 2274, 89-100, May 2021.
• 董 金華, 上田 宏. 転写因子IRF4の欠失によるCD4+ T細胞の機能不全が移植受容を促進する, 実験医学, 羊土社, Vol. 36, No. 4, p. 546-547, Mar. 2018.
• Jinhua Dong, Hiroshi Ueda. Open sandwich immunoassay and its applications, Advances in Medicine and Biology, Nova Science Publishers Inc., New York, Vol. 125, pp. 123-159, Oct. 2017.
• 生物化学的測定研究会, 上田宏, 小林典裕, 三宅司郎, 荒川秀俊(編). 免疫測定法 基礎から先端まで, 免疫測定法 基礎から先端まで, 講談社, Dec. 2014.

国際会議発表 (査読有り)

• 上田宏, 大室有紀. Functional complementation of luciferase half-reactions: application to a robust protein-protein interaction assay FlimPIA, 18th International Symposium on Bioluminescence and Chemiluminescence, Luminescence, Wiley, Vol. 29, No. Suppl. 1, p. 50, June 2014.  公式リンク  
  

国内会議発表 (査読有り)

• 上田宏, 蘇九龍, 朱博, 井上暁人, 大山浩之, 森田いずみ, 董金華, 小西良子, 北口哲也, 小林典裕, 三宅司郎. 各種食中毒から生活者を守る「パトロール酵母」の開発, 第74回日本生物工学会大会要旨集, Oct. 2022.
• 上田 宏. 蛋白質工学の基礎技術：蛋白質機能の設計と分子進化, 第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会, 第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会要旨集, June 2019.
• 大室有紀, 五味恵子, 山地秀樹, 上田宏. 蛋白質間相互作用検出系FlimPIAにおけるプローブの熱安定性向上, 酵素工学研究会, Oct. 2015.
• 上田宏, 五味恵子, 山地秀樹, 大室有紀. ヘイケボタル酵素を用いた蛋白質間相互作用検出系FlimPIAの構築, 生物発光化学発光研究会, Oct. 2015.

国際会議発表 (査読なし・不明)

• Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Hiroshi Ueda, Tetsuya Kitaguchi. A one-pot detection system using β-glucuronidase-based enzyme switch and label-free antibody, The Joint Congress of 21st International Union for Pure and Applied Biophysics, The Joint Congress of 21st International Union for Pure and Applied Biophysics, June 2024.
• Takanobu Yasuda, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi. A bioluminescent immunosensor based on “Trap & Release” of luciferase-derived peptide fused to antibody, The Joint Congress of 21st International Union for Pure and Applied Biophysics, The Joint Congress of 21st International Union for Pure and Applied Biophysics, June 2024.
• Yinghui Yang, Akihito Inoue, Takanobu Yasuda, Hiroshi Ueda, Bo Zhu, Tetsuya Kitaguchi. Fabrication of a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing, The Joint Congress of 21st International Union for Pure and Applied Biophysics, The Joint Congress of 21st International Union for Pure and Applied Biophysics, June 2024.
• Cheng Qian, Ayumu Ninomiya, Natsuki Shibukawa, Hiroshi Ueda, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi. PMBiT: A bioluminescent probe for large antigen detection, The Joint Congress of 21st International Union for Pure and Applied Biophysics, The Joint Congress of 21st International Union for Pure and Applied Biophysics, June 2024.
• Kana Sasamoto, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda. Development of “Coiled Q-probe” that can effectively convert antibodies into fluorescent immunosensors, 33rd Anniversary World Congress on Biosensors, 33rd Anniversary World Congress on Biosensors, June 2023.
• Akihito Inoue, Takanobu Yasuda, Tetsuya Kitaguchi, Akikazu Murakami, Hiroshi Ueda.. Selection and Trp-mutation evolution of a nanobody-based fluorescent immunosensor "nano Q-body" by yeast surface display, 33rd Anniversary World Congress on Biosensors, 33rd Anniversary World Congress on Biosensors, June 2023.
• Hiroshi Ueda, Bo Zhu, Jiulong Su, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake.. The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning, 33rd Anniversary World Congress on Biosensors, 33rd Anniversary World Congress on Biosensors, June 2023.
• Hiroshi Ueda, Bo Zhu, Jiulong Su, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake.. Development of “patrol yeasts” for the detection of multiple toxic substances in foods, The 15th Asian Congress on Biotechnology, The 15th Asian Congress on Biotechnology, Oct. 2022.
• Hiroshi Ueda, Bo Zhu, Jinhua Dong. Isolation of recombinant human antibody for the prevention and rapid diagnosis of COVID-19, Joint SBJ Meeting with Indonesia, Philippines, and Thailand, Oct. 2022.
• Bo Zhu, Haimei Li, Yancen Dai, Akihito Inoue, Yinghui Yang, Kaori Kobayashi, Tetsuya Kitaguchi, Jinhua Dong, Hiroshi Ueda. Rapid construction of homogeneous immunosensors for SARS-CoV-2 nucleocapsid and spike proteins using Quenchbody and Quenchprobe technology, Bioanalytical Sensors Gordon Research Conference, Bioanalytical Sensors Gordon Research Conference, June 2022.
• Akihito Inoue, Yuki Ohmuro, Tetsuya Kitaguchi, Hiroshi Ueda. Construction of mini Q-body using the nanobody to detect chemotherapy agent., Nonobodies (Hybrid 2nd edition), Sept. 2021.
• Hiroshi Ueda, Jinhua Dong, Takanobu Yasuda, Masaki Takahashi, Kana Sasamoto, Hiroyuki Oyama, Izumi Morita, Hee-Jin Jeong, Tetsuya Kitaguchi, Norihiro Kobayashi.. PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor., 31st Anniversary World Congress on Biosensors, July 2021.
• Akihito Inoue, Yuki Ohmuro, Tetsuya Kitaguchi, Hiroshi Ueda. Construction of mini Q-body using the nanobody to detect chemotherapy agent., 31st Anniversary World Congress on Biosensors, July 2021.
• Yuki Ohmuro, Tadaomi Furuta, Hayato Matsui, Masaki Kanai, Hiroshi Ueda. Development of the smallest luciferase “picALuc” among bright-light emitting luciferases, 2021 online symposium of the 21st ISBC & XIX ISLS, June 2021.
• Cyrus Beh, Theresa Seah, Jiulong Su, Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Hiroshi Ueda, Shawn Hoon.. Facile method for creating arrays of lipid-capped elastomer microwells for bioinspired protocellular sensors., µTAS 2019, Oct. 2019.
• Jiulong Su, Cyrus Beh, Tetsuya Kitaguchi, Yuki Ohmuro, Theresa Seah, Shawn Hoon, Hiroshi Ueda.. Creation of a novel sensor enzyme and its integration into protocell for digital detection of various anti-epitope tag antibodies., 18th Asian Pacific Confederation of Chemical Engineering Congress (APCChE 2019), Sept. 2019.
• 上田宏. 新規免疫測定法開発におけるBLIの活用例, 日本ポールユーザーミーティング2018, 日本ポールユーザーミーティング2018, May 2018.
• Hiroshi Ueda, Yuki Ohmuro. Homogeneous noncompetitive immunological detection of small molecules by ternary luciferase fragment complementation (OS-BLIA), 20th International Symposium on Bioluminescence and Chemiluminescence, May 2018.
• Hiroshi Ueda. Development of antibody-based imaging probes utilizing fluorescence quenching phenomena, International Conference on Translational Medicine and Imaging(ICTMI) 2017, Aug. 2017.
• Hiroshi Ueda, Yuya Oka, Chan-I Chung, Hee-Jin Jeong, Jinhua Dong. Antibody-based Fluorescent Probes: How Can We Convert Antigen Binding Signal to Fluorescence Signal?, Third FB3 (Fluorescent Biomolecules and their Building Blocks) Conference, July 2016.
• Masaki Muto, Kentaro Yanai, Masayoshi Tanaka, Hiroshi Ueda, Takeshi Onodera, Kiyoshi Toko, Mina Okochi. Construction of peptide probe for detection of volatile explosive compounds, Biosensors 2016, May 2016.
• Hiroshi Ueda. Development of biosensor Proteins Utilizing Fluorescence Quenching and Bioluminescent Reactions, International Conference on Biomolecular Engineering（ICBE)2016, Jan. 2016.
• Hiroshi Ueda, Hee-Jin Jeong, Takuya Kawamura, Manami Iida, Yumi Kawahigashi, Mutsumi Takigawa, Chan-I Chung, Jinhua Dong, Masuo Kondoh. Development of Quenchbody to image and quantify tumor-associated membrane protein claudin, Pacifichem 2015, Nov. 2015.
• Hiroshi Ueda, Hideki Yamaji, Yuki Ohmuro-Matsuyama. Hinge region engineering of firefly luciferases for robust protein-protein interaction assay FlimPIA, Pacifichem 2015, Nov. 2015.
• Shitao Zhao, Hee-Jin Jeong, Koichi Okumura, Hiroshi Ueda. Fast and sensitive detection of a neonicotinoid insecticide imidacloprid with a reagentless immunoassay reagent Quenchbody, Pacifichem 2015, Nov. 2015.
• Chan-I Chung, Yuki Ohmuro-Matsuyama, Yuko Sato, Hiroshi Kimura, Hiroshi Ueda. Development of intrabody-based FRET probe for monitoring histone H3 acetylation in living cells, Pacifichem 2015, Nov. 2015.
• 上田宏. Quenchbodies - Quench-based antibody probes that emit antigen-dependent fluorescence, International Conference and Exhibition on Antibodies, Immunome Research, OMICS International, vol. 11, no. 2, p. 49, Aug. 2015.
• Hiroshi Ueda, Hee-Jin Jeong, Yuki Ohmuro-Matsuyama, Jinhua Dong. Development of Biosensor Proteins Utilizing Fluorescence Quenching and Bioluminescent Reactions, 2015 International Symposium & Annual Meeting, KMB, Program, 2015 International Symposium & Annual Meeting, KMB, June 2015.
• Hiroshi Ueda, Takahiro Yamashita, Yuki Ohmuro-Matsuyama. Functional Complementation of Luciferase Half-Reactions: Application to a Robust Protein-Protein Interaction Assay FlimPIA, Active Enzyme Molecules 2014, Dec. 2014.
• Eizo Miyashita, Hiroshi Ueda, Yuki Ueyama. The monkey primary motor cortex may generate motor commands and estimated arm states during reaching movements, International Symposium on Application of Biomechanical Control Systems to Precision Engineering, pp. 74–75, 2010.

国内会議発表 (査読なし・不明)

• Li Tian, Keisuke Yamashita, Takanobu Yasuda, Takashi Tsuboi, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi. Single fluorescent protein-based biosensor for monitoring Intracellular IP3 dynamics, 化学工学会第89年会 要旨集, Mar. 2024.
• 小野義光, 有坂亮汰, 二宮歩武, 柴田貴広, 内田浩二, 安田貴信, 朱博, 董金華, 上田宏, 北口哲也. ゲノム編集によるオープンサンドイッチ免疫測定用抗体の構築と均一測定系への展開, 化学工学会第89年会, 化学工学会第89年会 要旨集, Mar. 2024.
• 渋川 夏希, 笹本 佳那, 瀬尾 秀宗, 安田 貴信, 朱 博, 太田 邦史, 上田 宏, 北口 哲也. バイオセンサーに変換可能な抗体の取得に向けた蛍光プローブタンパク質の開発, 化学工学会第89年会, 化学工学会第89年会 要旨集, Mar. 2024.
• 安田 貴信, 朱 博, 上田 宏, 北口 哲也. 発光酵素断片のトラップ&リリースに基づく抗原検出法, 化学工学会第89年会, 化学工学会第89年会 要旨集, Mar. 2024.
• Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Hiroshi Ueda, Tetsuya Kitaguchi. A customizable one-pot detection system using β-glucuronidase switch and label-free antibody, 化学工学会第89年会, 化学工学会第89年会 要旨集, Mar. 2024.
• 笹本佳那, 安田貴信, 朱博, 上田宏, 北口哲也. 順平行コイルドコイルペプチド型蛍光プローブによる蛍光免疫センサの応答向上, 第12回日本生物工学会東日本支部コロキウム 要旨集, Feb. 2024.
• 安田貴信, 朱博, 上田宏, 北口哲也. 生物発光免疫センサーの発光活性増大機構の解明とその原理の 全長抗体への展開, 第2回日本抗体学会学術大会, 第2回日本抗体学会学術大会要旨集, Dec. 2023.
• Zixu Feng, 相原寛史, 桜庭俊, Yanyan Hou, 吉田啓亮, 安田貴信, Bo Zhu, 上田宏, 伊藤寿朗, 北口哲也. 生細胞イメージングに適用可能なジベレリン蛍光免疫センサーへの改良, 第2回日本抗体学会学術大会, 第2回日本抗体学会学術大会要旨集, Dec. 2023.
• Yinghui Yang, Akihito Inoue, Takanobu Yasuda, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi. Fabrication of Nanobody-based Ratiometric Bioluminescent Quenchbody for Simple and Rapid Detection, 第75回日本生物工学会大会 要旨集, Sept. 2023.
• Zhu, B., Qiu, Z., Mizutani, K., Kobayashi, K., Ueda, H., Kitaguchi, T.. Rapid development of single fluorescent protein-based immunosensor Flashbody through ultra-high-throughput screening and cell-free protein synthesis, 第75回⽇本⽣物⼯学会⼤会, 第75回⽇本⽣物⼯学会⼤会 要旨集, Sept. 2023.
• 安田貴信, 朱博, 上田宏, 北口哲也. 抗体結合タンパク質を利用した全長抗体の生物発光センサー化, 化学工学会第54回秋季大会, 化学工学会第54回秋季大会要旨集, Aug. 2023.
• 大室有紀, 北口哲也, 上田宏, 木村宏. YFP断片とintrabodyを利用した生細胞内におけるエピジェネティクス変化の観察法構築, 第45回日本分子生物学会年会, 第16回日本エピジェネティクス研究会年会 要旨集, June 2023.
• 佐藤 里杏人, 安田 貴信, 朱 博, 北口 哲也, 加藤 直洋, 上田 宏. ３分割ルシフェラーゼを用いた免疫測定法における相互作用の測定と数理モデルの作製, 化学工学会第88年会, 化学工学会第88年会要旨集, Mar. 2023.
• 井上暁人, 安田貴信, 朱博, 北口哲也, 村上明一, 上田宏. 酵母細胞表層提示系を用いたナノ抗体由来”nano Q-body”のTrp変異進化, 化学工学会第88年会, 化学工学会第88年会要旨集, Mar. 2023.
• 笹本佳那, 安田貴信, 北口哲也, 上田宏. 逆並行結合抑制型ペプチドペアによる抗体を蛍光免疫センサに変換するプローブタンパク質Coiled Q-probeの蛍光応答向上, 第95回日本生化学会大会, 第95回日本生化学会大会要旨集, Nov. 2022.
• Keisuke Yamashita, Takashi Tsuboi, Hiroshi Ueda, Tetsuya Kitaguchi.. Development of a GFP-based biosensor for visualizing intracellular IP3 dynamics, 第45回日本分子生物学会年会, 第45回日本分子生物学会年会要旨集, Nov. 2022.
• 日浅 綜平, 藤森 武流, Saki Aiki, 上田 宏, Takashi Tsuboi, 北口 哲也. 幅広い濃度範囲の検出を可能とする複数の緑色蛍光cAMPセンサーの開発, 第45回日本分子生物学会年会, 第45回日本分子生物学会年会要旨集, Nov. 2022.
• 井上暁人, 安田貴信, 北口哲也, 村上明一, 上田宏. 酵母提示系によるナノ抗体由来の蛍光免疫センサnano Q-bodyの分子進化法の開発, 第95回日本生化学会大会, 第95回日本生化学会大会要旨集, Nov. 2022.
• 塘 知子, 朱 博, 北口 哲也, 上田 宏. 蛍光免疫センサーQuenchbodyのシグナル増幅と高速スクリーニング法の探索, 第95回日本生化学会大会, 第95回日本生化学会大会要旨集, Nov. 2022.
• 大室（松山）有紀, 古田忠臣, 松井勇人, 叶井正樹, 上田宏. 最小サイズの発光酵素「picALuc」の開発とその応用, 創立100周年記念第74回日本生物工学会大会, Oct. 2022.
• 伊藤良浩, 佐々木隆一, 朝里さやか, 北口哲也, 上田 宏. 微小ドロップレット培養とバイオセンサーを用いたタンパク質高生産株スクリーニング法の開発, 第74回日本生物工学会大会, 第74回日本生物工学会大会要旨集, Oct. 2022.
• 倉田晴生, 安田貴信, 北口哲也, 上田宏. 発光タグを付加したペプチドを用いた免疫センサーの構築, 化学工学会第53回秋季大会, 化学工学会第53 回秋季大会要旨集, Sept. 2022.
• 安田貴信, 笹本佳那, 北口哲也, 上田宏. 逆平行結合抑制ペプチドペアを用いたクエンチ抗体の構築と評価, 第16回バイオ関連化学シンポジウム, 第16回バイオ関連化学シンポジウム要旨集, Sept. 2022.
• 井上暁人, 鈴木大凱, YANG Yinghui, 安田貴信, 朱博, 北口哲也, 上田宏. コルチゾール認識ナノ抗体の蛍光免疫センサー化, 第16回バイオ関連化学シンポジウム, 第16回バイオ関連化学シンポジウム要旨集, Sept. 2022.
• 朱博, 野坂 宜之, 董 金華, 北口 哲也, 小池 竜 司, 若林 健二, 上田 宏. Converting antibodies to rapid response SARS-CoV-2 homogeneous immunosensors via Quenchbody technology and crowding agent, 化学工学会第53回秋季大会, 化学工学会第53 回秋季大会要旨集, Sept. 2022.
• 上田 宏, DAI YANCEN, 佐藤 優子, 朱 博, 北口 哲也, 木村 宏, Farid J. Ghadessy. 生細胞内タンパク質の動態観察とそれによる細胞分別が可能な蛍光抗体プローブIntra Q-bodyの構築, 第16回バイオ関連化学シンポジウム 要旨集, Sept. 2022.
• Cheng Qian, Bo Zhu, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda. Development of a thermostable beta-glucuronidase enzyme switch by introducing mutations that weaken interactions between diagonal subunits, 2022年度生物工学若手研究者の集い 要旨集, May 2022.
• 大室有紀, 古田忠臣, 松井勇人, 叶井正樹, 上田宏. 発光酵素の特性を利用したタンパク質間相互作用検出系、及び新規小型発光酵素の開発, 日本顕微鏡学会第78回学術講演会, May 2022.
• Marie Mita, Izumi Sugawara, Kazuki Harada, Motoki Ito, Mai Takizawa, Kentaro Ishida, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi.. Visualization of intracellular glucose metabolism dynamics by red fluorescent protein-based glucose indicators., 第99回日本生理学会大会, 第99回日本生理学会大会要旨集, Mar. 2022.
• 小林香央里, 朱博, 北口哲也, 上田宏. 蛍光免疫プローブQuenchprobeのダブルラベル化の検討, 化学工学会第87年会, 化学工学会第87年会要旨集, Mar. 2022.
• Yancen Dai, Bo Zhu, Yuko Sato, Hiroshi Kimura, Farid J. Ghadessy, Tetsuya Kitaguchi, Hiroshi Ueda.. Development of an immunofluorescence probe Q-body for the direct visualization of tumor marker p53 in fixed and living cells., 化学工学会第87年会, 化学工学会第87年会要旨集, Mar. 2022.
• Yanmin Chen, Yancen Dai, Tetsuya Kitaguchi, Hiroshi Ueda.. Development of a multicolor Q-body construction method using Escherichia coli., 化学工学会第87年会, 化学工学会第87年会要旨集, Mar. 2022.
• 椿凜果, 安田貴信, 朱博, 北口哲也, 上田宏. 蛍光免疫センサーCoiled Quenchbodyのがん光線力学療法への応用, 化学工学会第87年会, 化学工学会第87年会要旨集, Mar. 2022.
• Yinghui Yang, Akihito Inoue, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda. Fabrication of BRET Q-body Using Various Antibody Fragments, 第10回日本生物工学会東日本支部コロキウム 要旨集, Mar. 2022.
• Ippei Tsujimura, Hiroshi Ueda, Tetsuya Kitaguchi.. Development of an antibody-based red fluorescent probe and its characterization., 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• 安田貴信, 上野慶行, 栃尾尚哉, 八木宏昌, 北口哲也, 木川隆則, 上田宏. NMRによる新規免疫センサーHibodyの抗原結合に伴う発光活性増大機構の解明, 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi.. Development of green fluorescent protein-based indicators visualizing wide-range of cAMP dynamics for live-cell imaging., 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• 三田真理恵, 菅原和, 原田一貴, 伊藤幹, 滝澤舞, 石田賢太郎, 上田宏, 北口哲也, 坪井貴司. 赤色蛍光タンパク質型グルコースセンサーの開発と糖代謝のライブセルイメージング, 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• 本城麻衣, 相原寛史, Yanyan Hou, 上田宏, 伊藤寿朗, 北口哲也. 抗体を分子認識ドメインとする蛍光タンパク質プローブを用いたジベレリンの定量, 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• Keisuke Yamashita, Ippei Tsujimura, Takashi Tsuboi, Hiroshi Ueda, Tetsuya Kitaguchi.. Generation of a genetically-encoded IP3 biosensor based on a green fluorescent protein., 第44回日本分子生物学会年会, 第44回日本分子生物学会年会要旨集, Dec. 2021.
• 飯島快斗, 上野慶行, 北口哲也, 上田宏. 発光タグ融合抗体結合タンパク質を用いた新規抗原検出系の構築, 第94回日本生化学会大会, 第94回日本生化学会大会要旨集, Nov. 2021.
• 笹本佳那, 安田貴信, 北口哲也, 上田宏. 蛍光免疫センサープローブCoiled Q-probeを用いた各種低分子の検出と，FRET型プローブへの展開, 第94回日本生化学会大会, 第94回日本生化学会大会要旨集, Nov. 2021.
• 安田貴信, 井上暁人, 北口哲也, 上田宏. レシオ計測が可能な蛍光免疫センサーFRET Coiled Q-bodyにおけるDonor-Acceptor間距離の検討, 第94回日本生化学会大会, 第94回日本生化学会大会要旨集, Nov. 2021.
• 大室有紀, 古田忠臣, 松井勇人, 叶井正樹, 上田宏. 明るい小型発光酵素 "picALuc", 生物発光化学発光研究会第36回学術講演会, Nov. 2021.
• 井上暁人, 安田貴信, 北口哲也, 村上 明一, 上田宏. 酵母提示系を用いたナノ抗体由来の蛍光免疫センサ“mini Q-body”選択法の開発, 第 73回日本生物工学会大会, 第 73回日本生物工学会大会要旨集, Oct. 2021.
• Yancen Dai, Atsushi Izutani, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda. Intracellular detection of tumor suppressor protein p53 by Q-body technology, 第 73回日本生物工学会大会, 第 73回日本生物工学会大会要旨集, Oct. 2021.
• 井上暁人, 安田貴信, 北口哲也, 村上 明一, 上田宏. 酵母細胞表層提示系を用いた抗ヒト血清アルブミン (HSA)ナノ抗体由来“mini Q-body”の選択, 化学工学会第52回秋季大会, 化学工学会第52回秋季大会要旨集, Sept. 2021.
• 朱博, 董金華, 寧雪じょう, 井上暁人, 北口哲也, 上田宏. Quenchbodyを用いた1分間SARS-CoV-2及び変異株の検出法, 化学工学会第52回秋季大会, 化学工学会第52回秋季大会要旨集, Sept. 2021.
• パクジュンテ, 有坂亮汰, 柴田貴広, 内田浩二, 董金華, 北口哲也, 上田宏. ゲノム編集法によるオープンサンドイッチ免疫測定用抗体の産生, 化学工学会第52回秋季大会, 化学工学会第52回秋季大会要旨集, Sept. 2021.
• 上田宏, 有坂亮汰, 朴俊泰, 董金華, 柴田貴広, 大室有紀, 北口哲也, 内田浩二. ゲノム編集法による酸化脂質類の非競合的免疫測定系の構築, 第74回日本酸化ストレス学会・第21回日本NO学会 合同学術集会, 第74回日本酸化ストレス学会・第21回日本NO学会 合同学術集会要旨集, May 2021.
• 笹本佳那, 安田貴信, 北口哲也, 上田宏. 迅速簡便に高性能な蛍光免疫センサーを構築可能なCoiled Q-probeの創出, 化学工学会第86年会, 化学工学会第86年会要旨集, Mar. 2021.
• Marie Mita, Mai Takizawa, Rei Nagata, Kazuki Harada, Hiroshi Ueda, Tetsuya Kitaguchi, Takashi Tsuboi.. Sucralose activates intracellular Ca2+ and cAMP signaling in enteroendocrine L cells., 第98回日本生理学会大会, 第98回日本生理学会大会要旨集, Mar. 2021.
• Zhu Bo, Dong Jinhua, Xuerao Ning, Akihito Inoue, Tetsuya Kitaguchi, Hiroshi Ueda.. Development of quenchbody-based 1-minute SARS-CoV-2 detection assay., 化学工学会第86年会, 化学工学会第86年会要旨集, Mar. 2021.
• Xuerao Ning, Tetsuya Kitaguchi, Hiroshi Ueda. Construction of fluorescent biosensor Q-body for detecting recombinant proteins produced in bioprocess, 化学工学会第86年会, 化学工学会第86年会要旨集, Mar. 2021.
• Yancen Dai, Atsushi Izutani, Takanobu Yasuda, Tetsuya Kitaguchi, Hiroshi Ueda.. Instant detection of tumor suppressor protein p53 by Q-body technology., 化学工学会第86年会, 化学工学会第86年会要旨集, Mar. 2021.
• Junqian Wang, Tetsuya Kitaguchi, Hiroshi Ueda. Development of a novel immunosensor system based on antigen-dependent assembly of fluorescent proteins., 化学工学会第86年会, 化学工学会第86年会要旨集, Mar. 2021.
• Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi.. Development of turn-on type green fluorescent cAMP biosensors applicable to wide range of cAMP dynamics., 第43回日本分子生物学会年会, 第43回日本分子生物学会年会要旨集, Dec. 2020.
• Jiulong Su, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda.. Development of patrol yeasts for the detection of toxic substances in foods., 第43回日本分子生物学会年会, 第43回日本分子生物学会年会要旨集, Dec. 2020.
• Ippei Tsujimura, Hiroshi Aihara, Hiroshi Ueda, Tetsuya Kitaguchi.. Development of a red fluorescent probe inducing its intensity change by antigen binding., 第43回日本分子生物学会年会, 第43回日本分子生物学会年会要旨集, Dec. 2020.
• Hiroshi Aihara, Yanyan Hou, Hiroshi Ueda, Toshiro Ito, Tetsuya Kitaguchi.. Development of a plant hormone probe employing antibody as molecular recognition domain for visualizing intracellular dynamics., 第43回日本分子生物学会年会, 第43回日本分子生物学会年会要旨集, Dec. 2020.
• 上田宏, 董金華, 三宅千絢, 安田貴信, 髙橋昌樹, 大山浩之, 森田いずみ, 北口哲也, 小林典裕. 抗体を蛍光免疫センサーQ-body 化可能なタンパク質PM Q-probe による各種バイオマーカーの測定, 化学工学会第51回秋季大会, 化学工学会第51回秋季大会要旨集, Sept. 2020.
• Jiulong Su, Yasuyuki Nakamura, Tetsuya Kitaguchi, Jun Ishii, Akihito Kondo, Norihiro Kobayashi, Shiro Miyake, Hiroshi Ueda.. Development of patrol yeasts for the detection of toxic substances in foods., 第93回日本生化学会大会, 第93回日本生化学会大会要旨集, Sept. 2020.
• 井上暁人, 安田貴信, 北口哲也, 上田宏. 蛍光免疫センサーQ-bodyを提示した酵母細胞の構築と抗原検出, 化学工学会第51回秋季大会, 化学工学会第51回秋季大会要旨集, Sept. 2020.
• Takanobu Yasuda, Akihito Inoue, Tetsuya Kitaguchi, Hiroshi Ueda.. Creation and evaluation of immunosensors that enable ratiometric assay by rapid dual-color labeling of antibody fragments., 第20回日本蛋白質科学会年会, 第20回日本蛋白質科学会年会要旨集, July 2020.
• Yoshiyuki Ueno, Naoya Tochio, Junqian Wang, Tetsuya Kitaguchi, Takanori Kigawa, Hiroshi Ueda.. Mechanistic analysis of novel immunosensor Hibody to clarify the antigen-dependent protrusion of tag sequence attached to the antibody N-terminus., 第20回日本蛋白質科学会年会, 第20回日本蛋白質科学会年会要旨集, July 2020.
• 有坂亮汰, 北口哲也, 上田宏. ハイブリドーマ細胞のゲノム編集によるオープンサンドイッチ免疫測定用人工抗体の生産, 化学工学会第85年会, 化学工学会第85年会要旨集, Mar. 2020.
• 上田 宏. タンパク質工学的手法による各種蛍光・発光免疫センサーの開発, 電気学会 光・量子デバイス研究会「医療・バイオ応用を目指したナノ構造・ナノ界面」, 電気学会 光・量子デバイス研究会「医療・バイオ応用を目指したナノ構造・ナノ界面」要旨集, Jan. 2020.
• 高橋昌樹, 董金華, 北口哲也, 上田宏. 抗体結合タンパク質Mからなる蛍光バイオセンサープローブ"Q-probe"の熱安定化と各種抗原検出への応用, 第42回日本分子生物学会年会, 第42回日本分子生物学会年会要旨集, Dec. 2019.
• 相原寛史, Yanyan Hou, 上田宏, 伊藤寿朗, 北口哲也. 生細胞イメージングを目指した遺伝子コード型ジベレリンプローブの開発, 第42回日本分子生物学会年会, 第42回日本分子生物学会年会要旨集, Dec. 2019.
• 千原貴美, 三田真理恵, 菅原和, 相木彩希, 早坂優希, 原田一貴, 上田宏, 北口哲也, 坪井貴司. 蛍光タンパク質を用いた緑色蛍光輝度変化型乳酸センサーの開発, 第42回日本分子生物学会年会, 第42回日本分子生物学会年会要旨集, Dec. 2019.
• 三田真理恵, 伊藤幹, 原田一貴, 菅原和, 永田怜, 芦田翼, 上田宏, 坪井貴司, 北口哲也. 緑色蛍光グルコースセンサーが検出する人工甘味料による膵β細胞のグルコース恒常性攪乱, 第42回日本分子生物学会年会, 第42回日本分子生物学会年会要旨集, Dec. 2019.
• 大室有紀, 北口哲也, 木村宏, 上田宏. YFP断片融合抗体プローブを用いたヒストン翻訳後修飾検出系の構築, 第 71回日本生物工学会大会, 第 71回日本生物工学会大会要旨集, Sept. 2019.
• 佐藤伸一, 松村雅喜, 上田宏, 中村浩之. チロシン残基修飾による抗体の部位選択的修飾と蛍光免疫センサー分子作成法への応用, 第13回バイオ関連化学シンポジウム, Sept. 2019.
• 上田宏, 安田貴信, 上野慶行, 井上暁人, 髙橋昌樹, 大室有紀, 北口哲也, 董金華. Beyond Q-body: 色素修飾が不要な新規免疫測定素子の構築, 第 71回日本生物工学会大会, 第 71回日本生物工学会大会要旨集, Sept. 2019.
• 上野慶行, 安田貴信, 大室有紀, 北口哲也, 上田宏. N末にNanoluc由来タグを持つ抗体断片は抗原依存的にNanolucの発光活性を増大させる, 第19回日本蛋白質科学会年会, 第19回日本蛋白質科学会年会要旨集, June 2019.
• 安田貴信, 北口哲也, 上田宏. コイルドコイル形成ペプチドを利用した蛍光ラベル化法による抗体断片の迅速FRETバイオセンサー化, 第19回日本蛋白質科学会年会, 第19回日本蛋白質科学会年会要旨集, June 2019.
• 大室有紀, 北口哲也, 木村宏, 上田宏. 分割YFP融合Intrabodyを用いた生細胞内ヒストンアセチル化検出プローブの構築, 第19回日本蛋白質科学会年会, 第19回日本蛋白質科学会年会要旨集, June 2019.
• 井上暁人, 大室有紀, 北口哲也, 上田宏. ナノ抗体を用いた抗がん剤センサーmini Q-body の構築, 第19回日本蛋白質科学会年会, 第19回日本蛋白質科学会年会要旨集, June 2019.
• 上田宏. ノーベル化学賞を受賞したイノベーター： Gregory Winter博士, 2019年度 日本生化学会関東支部例会, June 2019.
• Jiulong Su, Juntae Park, Hiroshi Ueda. Improved stability and lower background signal of sensitive immunosensor system based on mutant β-glucuronidase, 第91回日本生化学会大会, 第91回日本生化学会大会要旨集, Sept. 2018.
• 高橋 里帆, 大室 有紀, 上田 宏. 自ら光るクエンチ抗体BRET Q-bodyの発光特性, 第12回バイオ関連化学シンポジウム, 第12回バイオ関連化学シンポジウム講演要旨集, Sept. 2018.
• 上田宏, 大室有紀, 北口哲也. 均相系測定を可能にする抗原応答性標識抗体の創製, 日本分析化学会第67年会, Sept. 2018.
• 上田宏. クエンチ抗体による環境中低分子の迅速高感度検出, 日本進化学会第20回大会, Aug. 2018.
• 上田宏. 蛍光消光現象と発光酵素を利用した新原理免疫測定法の開発, 岡山県医用工学研究会第112回セミナー, Oct. 2017.
• 上田宏, 三宅千絢, 塚原知也, 高橋昌樹, 児島智樹, 鄭熙陳, 董金華, 北口哲也, 大橋広之. 蛍光免疫センサーQ-body迅速構築のための新しい戦略, 化学工学会第49回秋季大会, 化学工学会第49回秋季大会要旨集, Sept. 2017.
• 高橋里帆, 大室有紀, 上田 宏. 発光酵素融合クエンチ抗体を用いた生物発光抗原検出系の構築, 第69回日本生物工学会大会, 第69回日本生物工学会大会講演要旨集, 3P-G053, Sept. 2017.
• 高橋里帆, 大室有紀, 上田 宏. 発光酵素融合クエンチ抗体を用いたBRET抗原検出系の構築, 第17回日本蛋白質科学会年会, 第17回日本蛋白質科学会年会要旨集, Vol. 17, p. 1P-139, May 2017.
• 矢内健太郎, 武藤正記, Jin Wang, 田中祐圭, 上田 宏, 小野寺 武, 都甲 潔, 大河内美奈. 爆発物検出に向けた抗体由来の分子認識ペプチドの探索, 化学工学会, Mar. 2017.
• 三宅 千絢, 鄭 煕陳, 大室 有紀, 董 金華, 上田 宏. 天然抗体をQuenchbody化するための抗体結合蛍光プローブの開発, 化学工学会第82年会, 化学工学会第82年会要旨集, Feb. 2017.
• 田中 絵美理, 鍾 蝉伊, 大室 有紀, 董 金華, 上田 宏. 生物発光共鳴エネルギー移動を利用した蛋白質認識抗体プローブの開発, 化学工学会第82年会, 化学工学会第82年会要旨集, Feb. 2017.
• 大室 有紀, 上田 宏, 山地 秀樹. リチウム添加による昆虫細胞からの抗体分泌生産の向上, 化学工学会第82年会, 化学工学会第82年会要旨集, Feb. 2017.
• 岡 裕也, 鄭 煕陳, 董 金華, 上田 宏. 蛍光抗体バイオセンサーQuenchbodyを用いたがん細胞検出と治療の試み, 化学工学会第82年会, 化学工学会第82年会要旨集, Feb. 2017.
• 田中絵美理, 鐘蝉伊, 大室有紀, 上田宏. 血清アルブミン検出のための生物発光抗体プローブの開発, 第89回日本生化学会大会, 第89回日本生化学会大会要旨集, Sept. 2016.
• 武藤正記, 矢内健太郎, 田中祐圭, 上田宏, Jin Wang, 小野寺武, 都甲 潔, 大河内美奈. 抗体相補性決定領域に基づいた揮発性爆発化合物の特異的ペプチドプローブの開発, 第68回 日本生物工学会大会, Sept. 2016.
• 上田 宏, 蘇九龍, 董 金華, 岩井 宏徒. 人工アロステリック酵素の創出による高感度ホモジニアス免疫測定法の開発, 第68回日本生物工学会大会, 第68回日本生物工学会大会要旨集, Aug. 2016.
• 岩井宏徒, 董金華, 上田宏. 円順列変異抗体断片Clampbodyを用いた抗原によるβ-ラクタマーゼの活性制御, 第16回日本蛋白質科学会年会, 第16回日本蛋白質科学会年会要旨集, June 2016.
• 武藤正記, 矢内健太郎, Jin Wang, 田中祐圭, 上田宏, 小野寺武, 都甲潔, 大河内美奈. 爆発物検出センサの開発に向けた抗体アミノ酸配列に基づく認識プローブの設計, 化学とマイクロ・ナノシステム学会 第33回研究会, Apr. 2016.
• 矢内健太郎, 武藤正記, Jin Wang, 田中祐圭, 上田宏, 小野寺武, 都甲潔, 大河内美奈. ペプチドアレイを用いた抗体由来TNT結合ペプチドの配列最適化, 化学工学会 第80年会, Mar. 2016.
• 武藤正記, 矢内健太郎, Jin Wang, 田中祐圭, 上田 宏, 小野寺 武, 都甲 潔, 大河内美奈. 抗TNT抗体の相補性決定領域に基づいた爆発物認識ペプチドの開発, 電気化学会第83回大会, Mar. 2016.
• 上田宏. 組み換え抗体を用いた酸化脂質検出系の構築, 第11回レドックス・ライフイノベーションシンポジウム, Mar. 2016.
• 矢内健太郎, 武藤正記, 田中祐圭, 上田宏, 大河内美奈. 抗体の相補性決定領域に基づいたTNT結合ペプチドの開発, 第4回日本生物工学会東日本支部コロキウム, Mar. 2016.
• 岡 裕也, 鄭 熙陳, 董 金華, 上田 宏. 蛍光免疫センサーQuenchbodyを用いたがん抗原蛋白質HER2の検出, 化学工学会第81年会, 化学工学会第81年会要旨集, Feb. 2016.
• Jiulong Su, Jinhua Dong, Hiroshi Ueda. Development of a novel homogeneous immunoassay using mutant beta-glucuronidase, 化学工学会第81年会, 化学工学会第81年会要旨集, Feb. 2016.
• 上田宏. 組換え抗体と発光酵素を利用した新規測定法の開発, 第４回ネオバイオ分子研究会, Jan. 2016.
• 董 金華, 座古 保, 上田 宏. 新規免疫測定素子UQ-bodyを用いたアミロイドβオリゴマーの蛍光検出, BMB2015, BMB2015要旨集, Nov. 2015.
• 鄭熙陳, 松本 健司, 板山 修也, 阿部 亮二, 董金華, 新藤充, 上田 宏. 抗体タンパク質からの蛍光免疫センサーQuenchbodyの構築, BMB2015, BMB2015要旨集, Nov. 2015.
• 大室有紀, 五味恵子, 山地秀樹, 上田宏. 蛋白質間相互作用検出系FlimPIAの実用化を目指したプローブの熱安定性向上, BMB2015, BMB2015要旨集, Nov. 2015.
• 鄭熙陳, 松本健司, 板山修也, 阿部亮二, 董金華, 新藤充, 上田宏. 光化学修飾法によるQuenchbodyの構築, 第９回バイオ関連化学シンポジウム, 第９回バイオ関連化学シンポジウム要旨集, Sept. 2015.
• 大河内 美奈, 武藤 正記, 田中 祐圭, 上田 宏, 小野寺 武, 都甲 潔, . TNT抗体の抗原結合部位解析に基づいたペプチドプローブの設計, 第9回バイオ関連化学シンポジウム, Sept. 2015.
• 上田宏. 抗原結合により光る抗体Q-body の開発とそのバイオ計測への応用, 第67回日本生物工学会大会, 第67回日本生物工学会大会要旨集, Sept. 2015.
• 飯田愛未, 川東 祐美, 滝川 睦美, 鍾 蝉伊, 董金華, 近藤昌夫, 上田 宏. がん関連膜蛋白質クローディン可視化のためのQuenchbodyの開発, 第９回バイオ関連化学シンポジウム, 第９回バイオ関連化学シンポジウム要旨集, Sept. 2015.
• 河村拓哉, 川東 祐美, 滝川 睦美, 鍾 蝉伊, 董金華, 近藤昌夫, 上田 宏. がん関連膜蛋白質クローディンを認識するQuenchbodyの開発, 第15回日本蛋白質科学会年会, 第15回日本蛋白質科学会年会要旨集, May 2015.
• 上田 宏, 阿部亮二, 大橋広行. 夢の免疫測定素子Quenchbody(Q-body)について－食品安全への応用を中心に, ”未来へのバイオ技術"勉強会「食品の合理的な安全性証明」, May 2015.
• 董 金華, ジョンヒジン, 上田 宏. アミノ転移反応を利用したQuenchbodyの構築, 第15回日本蛋白質科学会年会, 第15回日本蛋白質科学会年会要旨集, May 2015.
• Hiroshi Ueda, Naoki Arai, Yuji Tamura, Eizo Miyashita. An alternative explanation of movement encoding in monkey's primary motor cortex using joint angular velocity and joint torque during reaching tasks, Neuro2010, Neuroscience Research, volume 68, Supplement 1, page e149, 2010.

その他の論文・著書など

• Yinghui Yang, Akihito Inoue, Takanobu Yasuda, Hiroshi Ueda, Bo Zhu, Tetsuya Kitaguchi. BRET nano Q-body: a nanobody-based ratiometric bioluminescent immunosensor for point-of-care testing, Jxiv, June 2024.
• Bo Zhu, Yukihiko Yamasaki, Takanobu Yasuda, Cheng Qian, Zhirou Qiu, Hiroshi Ueda, Tetsuya Kitaguchi. Customizable OpenGUS immunoassay: a homogeneous detection system using split β-glucuronidase and label-free antibody, Jxiv, Sept. 2023.
• 安田 貴信, 大森 健太, 上田宏, 北口哲也. トラップ&リリースが拓いた免疫センサーの道と抗体薬物複合体の未来, 生物工学会誌, 公益社団法人 日本生物工学会, Vol. 101, No. 6, pp. 277-280, June 2023.  公式リンク  
  
• Abdul Qawee Rani, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi. Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering, ANALYST, 148, 1422-1429, Mar. 2023.
• 伊藤良浩, 佐々木隆一, 朝里さやか, 北口哲也, 上田 宏. 微小ドロップレット培養とバイオセンサーを用いたタンパク質高生産株スクリーニング法の開発, 創立100周年記念 第74回日本生物工学会大会 トピックス集, 22-23, Oct. 2022.
• Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi, Tetsuya Kitaguchi. Development of green fluorescent protein-based cAMP indicators with expanded dynamic range and various working ranges, Jxiv, June 2022.
• Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi, Hiroshi Ueda.. Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces, bioRxiv, May 2022.
• Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami, Hiroshi Ueda.. Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface., Res Sq, Nov. 2021.
• Hiroshi Ueda, Jinhua Dong. Biosensors: Homogeneous Detection, Reference Module in Biomedical Sciences, May 2021.  公式リンク  
  
• 上田 宏. 抗体関連タンパク質の蛍光修飾によるバイオセンサー化, 生物工学会誌, 日本生物工学会, Vol. 99, No. 4, p. 168-171, Apr. 2021.
• Jinhua Dong, Hiroshi Ueda. Recent Advances in Quenchbody, a Fluorescent Immunosensor, Sensors, MDPI, vol. 21, Issue 4, p. 1223, Feb. 2021.  公式リンク  
  
• 上田宏. 2018年ノーベル化学賞を受賞したGregory Winter博士, 現代化学, 東京化学同人, No. 573, 26-27, Nov. 2018.
• Yuki Ohmuro-Matsuyama, Hiroshi Ueda. A Novel Protein-Protein Interaction Assay Based on the Functional Complementation of Mutant Firefly Luciferases: Split Structure Versus Divided Reaction, Protein-Protein Interaction Assays, IntechOpen, pp. 11-27, July 2018.  公式リンク  
  
• 董 金華, 上田 宏. FRETセンサーを用いた生細胞膜電位の定量的測定, 実験医学, 羊土社, Vol. 35, No. 9, p. 1464-5, May 2017.
• 董金華, 上田宏. CRISPR/Cas9システムによるマウス・ヒト抗体遺伝子座の編集, 実験医学, 羊土社, vol. 34, no. 9, May 2016.  公式リンク  
  
• 上田宏. 抗原結合により光る抗体Q-bodyの創出, 細胞, 北隆館/ニュー・サイエンス社, vol. 48, p. 23-27, Apr. 2016.  公式リンク  
  
• 阿部 亮二, 上田 宏. 「その場」検出を可能とする光る抗体Q-body, 検査技術, 日本工業出版, vol. 21, p. 9-15, Apr. 2016.  公式リンク  
  
• 上田宏. 抗体のセンサー化で生命現象を探る, 日経バイオテク12月21日号「バイオイメージング最前線」（第8回）, 日経BP社, Dec. 2015.  公式リンク  
  
• Hiroshi Ueda. Improving Medical Care with Engineering, SCIENTIA, Knowledge Translation Media, Gloucester, UK, Oct. 2015.  公式リンク  
  
• 董 金華, 上田 宏. 生物発光タンパク質センサーを用いた薬剤のモニタリング, 実験医学, 羊土社, vol. 33, no. 4, p. 583-584, Apr. 2015.
• 阿部 亮二, 上田 宏. 抗体断片の蛍光技術を用いた迅速・簡便な検査キットの開発, 最先端バイオマーカーを用いた診断薬／診断装置開発と薬事対応, （株）技術情報協会, 東京, p. 335-338, Jan. 2015.  公式リンク  
  
• 上田宏. 免疫反応を測れる 光る抗体, 現代化学, 東京化学同人, no. 518, p. 32-36, Apr. 2014.  公式リンク  
  
• 大室有紀, 上田 宏. ホタル発光酵素の反応機構を利用したタンパク質間相互作用検出系の開発, バイオサイエンスとインダストリー, バイオインダストリー協会, vol. 72, no. 2, p. 113-116, Mar. 2014.  公式リンク  
  

特許など

• 上田宏, 北口哲也, SuJiulong, 三宅　司郎　, 小林　典裕　. 抗体断片を含む人工受容体を細胞膜に提示した酵母とこれを用いた抗原検出法 . 特許. 公開. 国立大学法人東京工業大学, 学校法人麻布獣医学園, 学校法人神戸薬科大学. 2020/08/31. 特願2020-145870. 2022/03/11. 特開2022-040923. 2022.
• 上田宏, 北口哲也, 大室有紀, 安田貴信, 井上暁人. 複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法. 特許. 登録. 国立大学法人東京工業大学. 2020/06/03. 特願2021-524873. 2020/10/12. WO 2020/246495. 特許第7461661号. 2024/03/27 2024.
• 上田宏, 北口哲也, 大室有紀, 安田貴信, 井上暁人. 複数の蛍光色素を結合させたペプチドを用いるホモジニアス免疫測定法. 特許. 公開. 国立大学法人東京工業大学. 2020/06/03. PCT/JP2020/021899. 2020/10/12. WO 2020/246495. 2020.
• 上田宏, 北口哲也, 大室有紀, 上野慶行. レポータータンパク質断片を付加した組換え抗体を用いるホモジニアス免疫測定法. 特許. 公開. 国立大学法人東京工業大学. 2020/05/22. PCT/JP2020/020281. 2020/12/03. WO 2020/241486. 2020.
• 上田宏, 綱　美香　, 原田 義孝　. 低分子化合物の簡易検出法. 特許. 公開. 国立大学法人東京工業大学, 日本製粉株式会社. 2020/03/26. 特願2020-056188. 2021/10/07. 特開2021-156695. 2021.
• 上田宏, SuJiulong, 北口哲也, 大室有紀. ホモジニアス免疫測定法に適した酵素変異体. 特許. 公開. 国立大学法人東京工業大学. 2020/01/23. PCT/JP2020/002264. 2020/08/13. WO 2020/162203. 2020.
• 佐藤伸一, 中村浩之, 上田宏. 蛍光標識抗体又は抗体断片. 特許. 公開. 国立大学法人東京工業大学. 2019/07/10. 特願2020-534145. 2021/08/19. 再表2020/026733. 2021.
• 上田宏, SuJiulong, 北口哲也, 大室有紀, Shawn Hoon, Cyrus Beh　. バイオセンサー. 特許. 登録. 国立大学法人東京工業大学, シンガポール科学技術研究庁. 2018/11/02. 特願2018-207624. 2020/05/07. 特開2020-068746. 特許第7169582号. 2022/11/02 2022.
• 上田宏, 大室有紀, 三宅千絢, 塚原知也. 抗原検出又は測定用キット. 特許. 登録. 国立大学法人東京工業大学. 2018/01/17. 特願2018-566814. 2019/12/12. 再表2018-147018. 特許第6983419号. 2021/11/26 2021.
• 上田宏, 董金華. 融合タンパク質及びそれを用いた抗原の検出方法. 特許. 登録. 国立大学法人東京工業大学. 2016/12/21. 特願2017-563741. 2018/11/15. 再表2017/130610. 特許第6873483号. 2021/04/23 2021.
• 上田宏, 鄭熙陳, 董金華, 阿部　亮二. 抗体のヌクレオチド結合部位（ＮＢＳ）を利用して蛍光標識された抗体. 特許. 公開. 国立大学法人東京工業大学, ウシオ電機株式会社. 2015/09/03. 特願2015-174075. 2017/03/09. 特開2017-049184. 2017.
• 上田宏, 大室　有紀, 黒澤　恵子. ルシフェラーゼ変異体及びそれを用いた分子間相互作用検出方法. 特許. 公開. 国立大学法人東京工業大学, キッコーマン株式会社. 2015/04/08. 特願2015-079444. 2015/11/26. 特開2015-211668. 2015.

研究ハイライト

• 上田宏, 大室 有紀, 和田 猛. ホタルが光る仕組みを利用した「たんぱく質の出会い」検出技術を開発. 2010～2013. JST先端計測分析技術・機器開発プログラムの一環として、東京工業大学 資源化学研究所の上田 宏　教授（前　東京大学 大学院工学系研究科　准教授）らの開発チームは、ホタル発光酵素の反応機構を利用して、た....
  

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